This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Frye, J. G.
Right arrow Articles by McClelland, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Frye, J. G.
Right arrow Articles by McClelland, M.

 Previous Article  |  Next Article 

Journal of Bacteriology, February 2005, p. 1485-1492, Vol. 187, No. 4
0021-9193/05/$08.00+0     doi:10.1128/JB.187.4.1485-1492.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Host Gene Expression Changes and DNA Amplification during Temperate Phage Induction{dagger}

Jonathan G. Frye,{ddagger} Steffen Porwollik, Felisa Blackmer, Pui Cheng, and Michael McClelland*

Sidney Kimmel Cancer Center, San Diego, California

Received 16 January 2004/ Accepted 10 November 2004

Salmonella enterica serovar Typhimurium LT2 harbors four temperate prophages. The lytic cycle of these phages was induced with hydrogen peroxide or mitomycin C. Microarray analysis was used to monitor the increase in phage genome copy number and the changes in RNA expression. Phage gene transcription was classified temporally, and host genes that responded to hydrogen peroxide, mitomycin C, or phage induction were also identified. A region of the serovar Typhimurium LT2 host genome encompassing hundreds of genes, flanking the Fels-1 lambdoid prophage, was amplified manyfold during lytic induction, presumably due to Fels-1 runoff replication prior to excision, a phenomenon termed escape replication. An excisionase (xis) mutant of Fels-1 also induced escape replication but did not get packaged. Gifsy-1, a lambdoid prophage that does not normally produce escape replication, did so after deletion of either its integrase or excisionase genes. Escape replication is probably widespread; large regions of host genome amplification were also observed after phage induction in serovar Typhimurium strains SL1344 and 14028s at the suspected integration site of prophage genomes.

* Corresponding author. Mailing address: Sidney Kimmel Cancer Center, 10835 Altman Row, San Diego, CA 92121. Phone: (858) 450-5990, ext. 280. Fax: (858) 550-3998. E-mail: mmcclelland{at}skcc.org.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

{ddagger} Present address: Antimicrobial Resistance Research Unit, ARS, SAA, USDA, Russell Research Center, Athens, GA 30605.

Journal of Bacteriology, February 2005, p. 1485-1492, Vol. 187, No. 4
0021-9193/05/$08.00+0     doi:10.1128/JB.187.4.1485-1492.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Gunderson, C. W., Boldt, J. L., Authement, R. N., Segall, A. M. (2009). Peptide wrwycr Inhibits the Excision of Several Prophages and Traps Holliday Junctions inside Bacteria. J. Bacteriol. 191: 2169-2176 [Abstract] [Full Text]  
  • Stanton, T. B., Humphrey, S. B., Sharma, V. K., Zuerner, R. L. (2008). Collateral Effects of Antibiotics: Carbadox and Metronidazole Induce VSH-1 and Facilitate Gene Transfer among Brachyspira hyodysenteriae Strains. Appl. Environ. Microbiol. 74: 2950-2956 [Abstract] [Full Text]  
  • Ravantti, J. J., Ruokoranta, T. M., Alapuranen, A. M., Bamford, D. H. (2008). Global Transcriptional Responses of Pseudomonas aeruginosa to Phage PRR1 Infection. J. Virol. 82: 2324-2329 [Abstract] [Full Text]  
  • Flanigan, A., Gardner, J. F. (2007). Interaction of the Gifsy-1 Xis Protein with the Gifsy-1 attP Sequence. J. Bacteriol. 189: 6303-6311 [Abstract] [Full Text]  
  • Champion, K., Higgins, N. P. (2007). Growth Rate Toxicity Phenotypes and Homeostatic Supercoil Control Differentiate Escherichia coli from Salmonella enterica Serovar Typhimurium. J. Bacteriol. 189: 5839-5849 [Abstract] [Full Text]  
  • Lindroos, H., Vinnere, O., Mira, A., Repsilber, D., Naslund, K., Andersson, S. G. E. (2006). Genome Rearrangements, Deletions, and Amplifications in the Natural Population of Bartonella henselae. J. Bacteriol. 188: 7426-7439 [Abstract] [Full Text]  
  • Poranen, M. M., Ravantti, J. J., Grahn, A. M., Gupta, R., Auvinen, P., Bamford, D. H. (2006). Global Changes in Cellular Gene Expression during Bacteriophage PRD1 Infection.. J. Virol. 80: 8081-8088 [Abstract] [Full Text]  
  • Kutsukake, K., Nakashima, H., Tominaga, A., Abo, T. (2006). Two DNA Invertases Contribute to Flagellar Phase Variation in Salmonella enterica Serovar Typhimurium Strain LT2. J. Bacteriol. 188: 950-957 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»