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Journal of Bacteriology, March 2005, p. 1849-1855, Vol. 187, No. 5
0021-9193/05/$08.00+0     doi:10.1128/JB.187.5.1849-1855.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

The Promoter Region Rather than Its Downstream Inverted Repeat Sequence Is Responsible for Low-Level Transcription of the Thermostable Direct Hemolysin-Related Hemolysin (trh) Gene of Vibrio parahaemolyticus

Yoshitsugu Nakaguchi1,2 and Mitsuaki Nishibuchi2*

Graduate School of Medicine,1 Center for Southeast Asian Studies, Kyoto University, Yoshida, Sakyo-ku, Kyoto, Japan2

Received 12 April 2004/ Accepted 28 November 2004

We determined the transcriptional start site of the thermostable direct hemolysin-related hemolysin gene (trh) of Vibrio parahaemolyticus by using a PCR-based method and identified the promoter. Mutagenic analysis indicated that the promoter-bearing region rather than its downstream inverted repeat sequence was responsible for the low-revel of trh transcription.

* Corresponding author. Mailing address: Center for Southeast Asian Studies, Kyoto University, 46 Shimoadachi-cho, Yoshida, Sakyo-ku, Kyoto 606-8501, Japan. Phone: 81-75-753-7367. Fax: 81-75-753-7350. E-mail: nisibuti{at}cseas.kyoto-u.ac.jp.

Journal of Bacteriology, March 2005, p. 1849-1855, Vol. 187, No. 5
0021-9193/05/$08.00+0     doi:10.1128/JB.187.5.1849-1855.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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