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Journal of Bacteriology, March 2005, p. 1875-1877, Vol. 187, No. 5
0021-9193/05/$08.00+0     doi:10.1128/JB.187.5.1875-1877.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Involvement of P1 Adhesin in Gliding Motility of Mycoplasma pneumoniae as Revealed by the Inhibitory Effects of Antibody under Optimized Gliding Conditions

Shintaro Seto,^1, Tsuyoshi Kenri,² Tetsuo Tomiyama,³ and Makoto Miyata^1,4*

Department of Biology, Graduate School of Science, Osaka City University,¹ PRESTO, JST, Sumiyoshi-ku, Osaka,⁴ Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Musashimurayama,² Tomiyama Laboratories, Nerima-ku, Tokyo, Japan³

Received 15 September 2004/ Accepted 30 November 2004

To examine the participation of P1 adhesin in gliding of Mycoplasma pneumoniae, we examined the effects of an anti-P1 monoclonal antibody on individual gliding mycoplasmas. The antibody reduced the gliding speed and removed the gliding cells from the glass over time in a concentration-dependent manner but had only a slight effect on nongliding cells, suggesting that the conformational changes of P1 adhesin and its displacement are involved in the gliding mechanism.

Present address: Department of Oral Microbiology, Meikai University School of Dentistry, Keyakidai, Sakado, Saitama 350-0283, Japan.

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