This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lee, S. K.
Right arrow Articles by Keasling, J. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lee, S. K.
Right arrow Articles by Keasling, J. D.

 Previous Article  |  Next Article 

Journal of Bacteriology, April 2005, p. 2793-2800, Vol. 187, No. 8
0021-9193/05/$08.00+0     doi:10.1128/JB.187.8.2793-2800.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Catabolite Repression of the Propionate Catabolic Genes in Escherichia coli and Salmonella enterica: Evidence for Involvement of the Cyclic AMP Receptor Protein

Sung Kuk Lee,1 Jack D. Newman,1 and Jay D. Keasling1,2*

Department of Chemical Engineering, University of California,1 Synthetic Biology Department, Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California2

Received 24 September 2004/ Accepted 3 January 2005

Previous studies with Salmonella enterica serovar Typhimurium LT2 demonstrated that transcriptional activation of the prpBCDE operon requires the function of transcription factor PrpR, sigma-54, and IHF. In this study, we found that transcription from the prpBCDE and prpR promoters was down-regulated by the addition of glucose or glycerol, indicating that these genes may be regulated by the cyclic AMP (cAMP)-cAMP receptor protein (CRP) complex. Targeted mutagenesis of a putative CRP-binding site in the promoter region between prpR and prpBCDE suggested that these genes are under the control of CRP. Furthermore, cells with defects in cya or crp exhibited reduced transcriptional activation of prpR and prpBCDE in Escherichia coli. These results demonstrate that propionate metabolism is subject to catabolite repression by the global transcriptional regulator CRP and that this regulation is effected through control of both the regulator gene prpR and the prpBCDE operon itself. The unique properties of the regulation of these two divergent promoters may have important implications for mechanisms of CRP-dependent catabolite repression acting in conjunction with a member of the sigma-54 family of transcriptional activators.

* Corresponding author. Mailing address: Department of Chemical Engineering, University of California, Berkeley, CA 94720-1462. Phone: (510) 642-4862. Fax: (510) 643-1228. E-mail: keasling{at}berkeley.edu.

Journal of Bacteriology, April 2005, p. 2793-2800, Vol. 187, No. 8
0021-9193/05/$08.00+0     doi:10.1128/JB.187.8.2793-2800.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ishida, Y., Kori, A., Ishihama, A. (2009). Participation of Regulator AscG of the {beta}-Glucoside Utilization Operon in Regulation of the Propionate Catabolism Operon. J. Bacteriol. 191: 6136-6144 [Abstract] [Full Text]  
  • Franchini, A. G., Egli, T. (2006). Global gene expression in Escherichia coli K-12 during short-term and long-term adaptation to glucose-limited continuous culture conditions. Microbiology 152: 2111-2127 [Abstract] [Full Text]  
  • Lee, S. K., Keasling, J. D. (2005). A Propionate-Inducible Expression System for Enteric Bacteria. Appl. Environ. Microbiol. 71: 6856-6862 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»