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Journal of Bacteriology, April 2005, p. 2827-2835, Vol. 187, No. 8
0021-9193/05/$08.00+0     doi:10.1128/JB.187.8.2827-2835.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Cytochrome Oxidase Deficiency Protects Escherichia coli from Cell Death but Not from Filamentation Due to Thymine Deficiency or DNA Polymerase Inactivation

Bernard Strauss,* Kemba Kelly, and Damian Ekiert

Center for Molecular Oncology and Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, Illinois

Received 27 October 2004/ Accepted 28 December 2004

Temperature-sensitive DNA polymerase mutants (dnaE) are protected from cell death on incubation at nonpermissive temperature by mutation in the cydA gene controlling cytochrome bd oxidase. Protection is observed in complex (Luria-Bertani [LB]) medium but not on minimal medium. The cydA mutation protects a thymine-deficient strain from death in the absence of thymine on LB but not on minimal medium. Both dnaE and {Delta}thy mutants filament under nonpermissive conditions. Filamentation per se is not the cause of cell death, because the dnaE cydA double mutant forms long filaments after 24 h of incubation in LB medium at nonpermissive temperature. These filaments have multiply dispersed nucleoids and produce colonies on return to permissive conditions. The protective effect of a deficiency of cydA at high temperature is itself suppressed by overexpression of cytochrome bo3, indicating that the phenomenon is related to energy metabolism rather than to a specific effect of the cydA protein. We propose that filamentation and cell death resulting from thymine deprivation or slowing of DNA synthesis are not sequential events but occur in response to the same or a similar signal which is modulated in complex medium by cytochrome bd oxidase. The events which follow inhibition of replication fork progression due to either polymerase inactivation, thymine deprivation, or hydroxyurea inhibition differ in detail from those following actual DNA damage.

* Corresponding author. Mailing address: Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, IL 60637. Phone: (773) 702-1628. Fax: (773) 702-3172. E-mail: bs19{at}uchicago.edu.

Journal of Bacteriology, April 2005, p. 2827-2835, Vol. 187, No. 8
0021-9193/05/$08.00+0     doi:10.1128/JB.187.8.2827-2835.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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