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Journal of Bacteriology, April 2005, p. 2912-2916, Vol. 187, No. 8
0021-9193/05/$08.00+0     doi:10.1128/JB.187.8.2912-2916.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Identification of rcnA (yohM), a Nickel and Cobalt Resistance Gene in Escherichia coli

Agnès Rodrigue,* Géraldine Effantin, and Marie-Andrée Mandrand-Berthelot

Unité de Microbiologie et Génétique, Unité Mixte de Recherche 5122, Centre National de la Recherche Scientifique, Institut National des Sciences Appliquées de Lyon, Université Lyon I, Villeurbanne, France

Received 11 October 2004/ Accepted 10 January 2005

We report here on the isolation and primary characterization of the yohM gene of Escherichia coli. We show that yohM encodes a membrane-bound polypeptide conferring increased nickel and cobalt resistance in E. coli. yohM was specifically induced by nickel or cobalt but not by cadmium, zinc, or copper. Mutation of yohM increased the accumulation of nickel inside the cell, whereas cells harboring yohM in multicopy displayed reduced intracellular nickel content. Our data support the hypothesis that YohM is the first described efflux system for nickel and cobalt in E. coli. We propose rcnA (resistance to cobalt and nickel) as the new denomination of yohM.

* Corresponding author. Mailing address: UMG-UMR 5122 CNRS-INSA-UCBL, batiment A. Lwoff, 10 rue Dubois, 69622 Villeurbanne Cedex, France. Phone: (33) 472447980. Fax: (33) 472431584. E-mail: agnes.rodrigue{at}insa-lyon.fr.

Journal of Bacteriology, April 2005, p. 2912-2916, Vol. 187, No. 8
0021-9193/05/$08.00+0     doi:10.1128/JB.187.8.2912-2916.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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