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Journal of Bacteriology, June 2006, p. 3757-3762, Vol. 188, No. 11
0021-9193/06/$08.00+0     doi:10.1128/JB.00038-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Isolation and Characterization of VceC Gain-of-Function Mutants That Can Function with the AcrAB Multiple-Drug-Resistant Efflux Pump of Escherichia coli

Govindsamy Vediyappan, Tatyana Borisova, and Joe A. Fralick*

Department of Microbiology and Immunology, School of Medicine, Texas Tech University Health Sciences Center, Lubbock, Texas 79430

Received 10 January 2006/ Accepted 1 March 2006

VceC is the outer membrane component of the major facilitator (MF) VceAB-VceC multiple-drug-resistant (MDR) efflux pump of Vibrio cholerae. TolC is the outer membrane component of the resistance-nodulation-division AcrAB-TolC efflux pump of Escherichia coli. Although these proteins share little amino acid sequence identity, their crystal structures can be readily superimposed upon one another. In this study, we have asked if TolC and VceC are interchangeable for the functioning of the AcrAB and VceAB pumps. We have found that TolC can replace VceC to form a functional VceAB-TolC MDR pump, but VceC cannot replace TolC to form a functional AcrAB-VceC pump. However, we have been able to isolate gain-of-function (gof) VceC mutants which can functionally interface with AcrAB. These mutations map to four different amino acids located at the periplasmic tip of VceC. Chemical cross-linkage experiments indicate that both wild-type and gof mutant VceC can physically interact with the AcrAB complex, suggesting that these gof mutations are not affecting the recruitment of VceC to the AcrAB complex but rather its ability to functionally interface with the AcrAB pump.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, School of Medicine, Texas Tech University Health Sciences Center, Lubbock, TX 79430. Phone: (806) 743-2555. Fax: (806) 743-2334. E-mail: joe.fralick{at}ttuhsc.edu.

Journal of Bacteriology, June 2006, p. 3757-3762, Vol. 188, No. 11
0021-9193/06/$08.00+0     doi:10.1128/JB.00038-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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