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Journal of Bacteriology, June 2006, p. 4573-4576, Vol. 188, No. 12
0021-9193/06/$08.00+0     doi:10.1128/JB.00089-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

The Synchrony Phenotype Persists after Elimination of Multiple GATC Sites from the dnaA Promoter of Escherichia coli

Terry G. Wilkinson II,1,{dagger} G. C. Kedar,1 Chi Lee,1 Elena C. Guzmán,2 Douglas W. Smith,3 and Judith W. Zyskind1*

Department of Biology, San Diego State University, San Diego, California 92182-4614,1 Departamento de Bioquímica y Biología Molecular y Genética, Facultad de Ciencias de Extremadura E-06080 Badajoz, Spain,2 Section of Molecular Biology, Division of Biological Sciences, University of California, San Diego, La Jolla, California 92093-03483

Received 17 January 2006/ Accepted 12 April 2006

To examine whether methylation of the GATC sites present in the dnaA promoter region is responsible for the strict temporal coordination of initiation events at oriC as measured by the synchrony of initiation, we introduced point mutations eliminating three (TGW1) and five (TGW2) of the six GATC sites present in the dnaA promoter region. All of the strains containing these mutations, including the one with five GATC sites eliminated, initiated chromosomal replication synchronously.

* Corresponding author. Mailing address: Department of Biology, San Diego State University, 5500 Campanile Drive, San Diego, CA 92182-4614. Phone: (858) 453-9233. Fax: (858) 453-9233. E-mail: jzyskind{at}sciences.sdsu.edu.

{dagger} Present address: Graduate School of Biomedical Sciences, University of Texas Medical Branch, Galveston, TX 77555-1050.

Journal of Bacteriology, June 2006, p. 4573-4576, Vol. 188, No. 12
0021-9193/06/$08.00+0     doi:10.1128/JB.00089-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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