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Journal of Bacteriology, July 2006, p. 5293-5298, Vol. 188, No. 14
0021-9193/06/$08.00+0     doi:10.1128/JB.00285-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Peptidoglycan Structure Analysis of Lactococcus lactis Reveals the Presence of an L,D-Carboxypeptidase Involved in Peptidoglycan Maturation

Pascal Courtin,1,{dagger} Guy Miranda,1,{dagger},{ddagger} Alain Guillot,1 Françoise Wessner,1 Christine Mézange,1 Elena Domakova,2 Saulius Kulakauskas,2 and Marie-Pierre Chapot-Chartier1*

Institut National de la Recherche Agronomique, Unité de Biochimie Bactérienne,1 Unité Bactéries Lactiques et Pathogènes Opportunistes, 78352 Jouy-en-Josas Cedex, France2

Received 24 February 2006/ Accepted 25 April 2006

Detailed structural analysis of Lactococcus lactis peptidoglycan was achieved by identification of its constituent muropeptides separated by reverse phase high-performance liquid chromatography. Modification of the classical elution buffer allowed direct and sensitive analysis of the purified muropeptides by matrix-assisted laser desorption ionization-time of flight mass spectrometry. The structures of 45 muropeptides were assigned for L. lactis strain MG1363. Analysis of the muropeptide composition of an MG1363 dacB mutant showed that the dacB-encoded protein has L,D-carboxypeptidase activity and is involved in peptidoglycan maturation.

* Corresponding author. Mailing address: INRA, Unité de Biochimie Bactérienne, Domaine de Vilvert, 78352 Jouy-en-Josas Cedex, France. Phone: 33 1 34 65 22 68. Fax: 33 1 34 65 21 63. E-mail: Marie-Pierre.Chapot{at}jouy.inra.fr.

{dagger} P. Courtin and G. Miranda contributed equally to the work.

{ddagger} Present address: Unité de Génomique et Physiologie de la Lactation, INRA, Jouy-en-Josas, France.

Journal of Bacteriology, July 2006, p. 5293-5298, Vol. 188, No. 14
0021-9193/06/$08.00+0     doi:10.1128/JB.00285-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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