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Journal of Bacteriology, July 2006, p. 5304-5307, Vol. 188, No. 14
0021-9193/06/$08.00+0     doi:10.1128/JB.00262-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Osmotic Upshift Transiently Inhibits Uptake via ABC Transporters in Gram-Negative Bacteria

M. A. Fox,1 J. P. White,1 A. H. F. Hosie,2 E. M. Lodwig,1 and P. S. Poole1*

School of Biological Sciences, University of Reading, Reading RG6 6AJ, United Kingdom,1 Department of Microbiology, King's College London Dental Institute, Floor 28, Guy's Tower, Guy's Campus, London SE1 9RT, United Kingdom2

Received 20 February 2006/ Accepted 8 May 2006

ATP-binding cassette transporters from several rhizobia and Salmonella enterica serovar Typhimurium, but not secondarily coupled systems, were inhibited by high concentrations (100 to 500 mM) of various osmolytes, an effect reversed by the removal of the osmolyte. ABC systems were also inactivated in isolated pea bacteroids, probably due to the obligatory use of high-osmolarity isolation media. Measurement of nutrient cycling in isolated pea bacteroids is impeded by this effect.

* Corresponding author. Mailing address: School of Biological Sciences, University of Reading, Whiteknights, P.O. Box 228, Reading RG6 6AJ, United Kingdom. Phone: (44) 118 378 8895. Fax: (44) 118 378 6671. E-mail: p.s.poole{at}reading.ac.uk.

Journal of Bacteriology, July 2006, p. 5304-5307, Vol. 188, No. 14
0021-9193/06/$08.00+0     doi:10.1128/JB.00262-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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