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Journal of Bacteriology, July 2006, p. 5325-5330, Vol. 188, No. 14
0021-9193/06/$08.00+0     doi:10.1128/JB.00104-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Regulation of the Helicobacter pylori Fe-S Cluster Synthesis Protein NifS by Iron, Oxidative Stress Conditions, and Fur

Praveen Alamuri, Nalini Mehta, Andrew Burk, and Robert J. Maier*

Department of Microbiology, The University of Georgia, Athens, Georgia 30602

Received 19 January 2006/ Accepted 25 April 2006

Transcription of both chromosomal and extrachromosomally introduced nifS was regulated (up-expressed) by oxygen or by supplemental iron conditions. This up-expression was not observed in a fur mutant strain background or when an iron chelator was added. Iron-bound Fur (but not apo-Fur) recognized the nifS promoter, and Fur bound significantly farther upstream (–155 bp to –190 bp and –210 to –240 bp) in the promoter than documented Helicobacter pylori Fur binding regions. This binding was stronger than Fur recognition of the flgE or napA promoter and includes a Fur recognition sequence common to the H. pylori pfr and sodB upstream areas. Studies of Fur-regulated genes in H. pylori have indicated that apo-Fur acts as a repressor, but our results demonstrate that iron-bound Fur activates (nifS) transcription.

* Corresponding author. Mailing address: Department of Microbiology, 527 Biological Sciences Bldg., The University of Georgia, Athens, GA 30602. Phone: (706) 542-2323. Fax: (706) 542-2674. E-mail: rmaier{at}uga.edu.

Journal of Bacteriology, July 2006, p. 5325-5330, Vol. 188, No. 14
0021-9193/06/$08.00+0     doi:10.1128/JB.00104-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Carpenter, B. M., Whitmire, J. M., Merrell, D. S. (2009). This Is Not Your Mother's Repressor: the Complex Role of Fur in Pathogenesis. Infect. Immun. 77: 2590-2601 [Full Text]  
  • Pflock, M., Bathon, M., Schar, J., Muller, S., Mollenkopf, H., Meyer, T. F., Beier, D. (2007). The Orphan Response Regulator HP1021 of Helicobacter pylori Regulates Transcription of a Gene Cluster Presumably Involved in Acetone Metabolism. J. Bacteriol. 189: 2339-2349 [Abstract] [Full Text]  


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