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Journal of Bacteriology, August 2006, p. 5450-5459, Vol. 188, No. 15
0021-9193/06/$08.00+0     doi:10.1128/JB.00275-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

UvrD Helicase Suppresses Recombination and DNA Damage-Induced Deletions{dagger}

Josephine Kang* and Martin J. Blaser

Departments of Microbiology and Medicine, New York University School of Medicine, and VA Medical Center, New York, New York

Received 21 February 2006/ Accepted 21 May 2006

UvrD, a highly conserved helicase involved in mismatch repair, nucleotide excision repair (NER), and recombinational repair, plays a critical role in maintaining genomic stability and facilitating DNA lesion repair in many prokaryotic species. In this report, we focus on the UvrD homolog in Helicobacter pylori, a genetically diverse organism that lacks many known DNA repair proteins, including those involved in mismatch repair and recombinational repair, and that is noted for high levels of inter- and intragenomic recombination and mutation. H. pylori contains numerous DNA repeats in its compact genome and inhabits an environment rich in DNA-damaging agents that can lead to increased rearrangements between such repeats. We find that H. pylori UvrD functions to repair DNA damage and limit homologous recombination and DNA damage-induced genomic rearrangements between DNA repeats. Our results suggest that UvrD and other NER pathway proteins play a prominent role in maintaining genome integrity, especially after DNA damage; thus, NER may be especially critical in organisms such as H. pylori that face high-level genotoxic stress in vivo.

* Corresponding author. Mailing address: Department of Medicine, New York University School of Medicine, 550 First Avenue, New York, NY 10016. Phone: (212) 263-6394. Fax: (212) 263-3969. E-mail: kangm01{at}med.nyu.edu.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, August 2006, p. 5450-5459, Vol. 188, No. 15
0021-9193/06/$08.00+0     doi:10.1128/JB.00275-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Guthlein, C., Wanner, R. M., Sander, P., Davis, E. O., Bosshard, M., Jiricny, J., Bottger, E. C., Springer, B. (2009). Characterization of the Mycobacterial NER System Reveals Novel Functions of the uvrD1 Helicase. J. Bacteriol. 191: 555-562 [Abstract] [Full Text]  
  • Wang, G., Maier, R. J. (2009). A RecB-Like Helicase in Helicobacter pylori Is Important for DNA Repair and Host Colonization. Infect. Immun. 77: 286-291 [Abstract] [Full Text]  
  • Kang, J., Blaser, M. J. (2008). Repair and Antirepair DNA Helicases in Helicobacter pylori. J. Bacteriol. 190: 4218-4224 [Abstract] [Full Text]  
  • Wang, G., Maier, R. J. (2008). Critical Role of RecN in Recombinational DNA Repair and Survival of Helicobacter pylori. Infect. Immun. 76: 153-160 [Abstract] [Full Text]  


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