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Journal of Bacteriology, September 2006, p. 6419-6424, Vol. 188, No. 17
0021-9193/06/$08.00+0     doi:10.1128/JB.00565-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Independent Control of Replication Initiation of the Two Vibrio cholerae Chromosomes by DnaA and RctB

Stéphane Duigou,1 Kristine G. Knudsen,2 Ole Skovgaard,2 Elizabeth S. Egan,1 Anders Løbner-Olesen,2 and Matthew K. Waldor1*

Department of Microbiology, Tufts University School of Medicine and Howard Hughes Medical Institute, 136 Harrison Ave., Boston Massachusetts 02111,1 Department of Life Sciences and Chemistry, Roskilde University, DK-4000, Roskilde, Denmark2

Received 21 April 2006/ Accepted 16 June 2006

Although the two Vibrio cholerae chromosomes initiate replication in a coordinated fashion, we show here that each chromosome appears to have a specific replication initiator. DnaA overproduction promoted overinitiation of chromosome I and not chromosome II. In contrast, overproduction of RctB, a protein that binds to the origin of replication of chromosome II, promoted overinitiation of chromosome II and not chromosome I.

* Corresponding author. Mailing address: Department of Microbiology, Tufts University School of Medicine and Howard Hughes Medical Institute, 136 Harrison Ave., Boston, MA 02111. Phone: (617) 636-2730. Fax: (617) 636-2723. E-mail: matthew.waldor{at}tufts.edu.

Journal of Bacteriology, September 2006, p. 6419-6424, Vol. 188, No. 17
0021-9193/06/$08.00+0     doi:10.1128/JB.00565-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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