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Journal of Bacteriology, September 2006, p. 6435-6439, Vol. 188, No. 17
0021-9193/06/$08.00+0     doi:10.1128/JB.00668-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Genetic Evidence for an Interaction of the UbiG O-Methyltransferase with UbiX in Escherichia coli Coenzyme Q Biosynthesis

Melissa Gulmezian,1 Haitao Zhang,2 George T. Javor,2 and Catherine F. Clarke1*

Department of Chemistry and Biochemistry and the Molecular Biology Institute, University of California, Los Angeles, California 90095-1569,1 Department of Biochemistry, Loma Linda University School of Medicine, Loma Linda, California 923542

Received 10 May 2006/ Accepted 22 June 2006

IS16 is a thiol-sensitive, Q-deficient mutant strain of Escherichia coli. Here, we show that IS16 harbors a mutation in the ubiG gene encoding a methyltransferase required for two O-methylation steps of Q biosynthesis. Complementation of IS16 with either ubiG or ubiXK-12 reverses this phenotype, suggesting that UbiX may interact with UbiG.

* Corresponding author. Mailing address: Department of Chemistry and Biochemistry, University of California—Los Angeles, 607 Charles E. Young Drive East, Los Angeles, CA 90095-1569. Phone: (310) 825-0771. Fax: (310) 206-5213. E-mail: cathy{at}chem.ucla.edu.

Journal of Bacteriology, September 2006, p. 6435-6439, Vol. 188, No. 17
0021-9193/06/$08.00+0     doi:10.1128/JB.00668-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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