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Journal of Bacteriology, September 2006, p. 6553-6560, Vol. 188, No. 18
0021-9193/06/$08.00+0     doi:10.1128/JB.00447-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Hypoxanthine Incorporation Is Nonmutagenic in Escherichia coli

Brian Budke and Andrei Kuzminov*

Department of Microbiology, University of Illinois at Urbana-Champaign, Urbana, Illinois

Received 31 March 2006/ Accepted 28 June 2006

Endonuclease V, encoded by the nfi gene, initiates removal of the base analogs hypoxanthine and xanthine from DNA, acting to prevent mutagenesis from purine base deamination within the DNA. On the other hand, the RdgB nucleotide hydrolase in Escherichia coli is proposed to prevent hypoxanthine and xanthine incorporation into DNA by intercepting the noncanonical DNA precursors dITP and dXTP. Because many base analogs are mutagenic when incorporated into DNA, it is intuitive to think of RdgB as acting to prevent similar mutagenesis from deaminated purines in the DNA precursor pools. To test this idea, we used a set of Claire Cupples' strains to detect changes in spontaneous mutagenesis spectra, as well as in nitrous acid-induced mutagenesis spectra, in wild-type cells and in rdgB single, nfi single, and rdgB nfi double mutants. We found neither a significant increase in spontaneous mutagenesis in rdgB and nfi single mutants or the double mutant nor any changes in nitrous acid-induced mutagenesis for rdgB mutant strains. We conclude that incorporation of deaminated purines into DNA is nonmutagenic.

* Corresponding author. Mailing address: B103 C&LSL, 601 South Goodwin Ave., Urbana, IL 61801-3709. Phone: (217) 265-0329. Fax: (217) 244-6697. E-mail: kuzminov{at}life.uiuc.edu.

Journal of Bacteriology, September 2006, p. 6553-6560, Vol. 188, No. 18
0021-9193/06/$08.00+0     doi:10.1128/JB.00447-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Rotman, E., Kuzminov, A. (2007). The mutT Defect Does Not Elevate Chromosomal Fragmentation in Escherichia coli Because of the Surprisingly Low Levels of MutM/MutY-Recognized DNA Modifications. J. Bacteriol. 189: 6976-6988 [Abstract] [Full Text]  


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