This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lepage, E.
Right arrow Articles by Serror, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lepage, E.
Right arrow Articles by Serror, P.

 Previous Article  |  Next Article 

Journal of Bacteriology, October 2006, p. 6858-6868, Vol. 188, No. 19
0021-9193/06/$08.00+0     doi:10.1128/JB.00421-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Comparative Genomic Hybridization Analysis of Enterococcus faecalis: Identification of Genes Absent from Food Strains{dagger}

E. Lepage,1 S. Brinster,1 C. Caron,2 Céline Ducroix-Crepy,3 L. Rigottier-Gois,1 G. Dunny,4 C. Hennequet-Antier,2 and P. Serror1*

Unité des Bactéries Lactiques et pathogènes Opportunistes,1 Mathématiques, Informatique et Génomes,2 Centre de Ressources Biologiques, INRA, Jouy-en-Josas, France,3 Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota4

Received 27 March 2006/ Accepted 6 July 2006

Enterococcus faecalis, a member of the natural microbiota of animal and human intestinal tracts, is also present as a natural contaminant in a variety of fermented foods. Over the last decade, E. faecalis has emerged as a major cause of nosocomial infections. We investigated the genetic diversity in 30 clinical and food isolates, including strains V583 and MMH594, in order to determine whether clinical and food isolates could be distinguished. Data were obtained using comparative genomic hybridization and specific PCR with a total of 202 probes of E. faecalis, selected using the available V583 genome sequence and part of the MMH594 pathogenicity island. The cognate genes encoded mainly exported proteins. Hybridization data were analyzed by a two-component mixture model that estimates the probability of any given gene to be either present or absent in the strains. A total of 78 genes were found to be variable, as they were absent in at least one isolate. Most of the variable genes were clustered in regions that, in the published V583 sequence, related to prophages or mobile genetic elements. The variable genes were distributed in three main groups: (i) genes equally distributed between clinical and dairy food isolates, (ii) genes absent from dairy food-related isolates, and (iii) genes present in MMH594 and V583 strains only. Further analysis of the distribution of the last gene group in 70 other isolates confirmed that six of the probed genes were always absent in dairy food-related isolates, whereas they were detected in clinical and/or commensal isolates. Two of them corresponded to prophages that were not detected in the cognate isolates, thus possibly extending the number of genes absent from dairy food isolates. Genes specifically detected in clinical isolates may prove valuable for the development of new risk assessment markers for food safety studies and for identification of new factors that may contribute to host colonization or infection.

* Corresponding author. Mailing address: Unité des Bactéries Lactiques et pathogènes Opportunistes, INRA, Jouy-en-Josas, France. Phone: 33 1 34 65 21 66. Fax: 33 1 34 65 20 65. E-mail: pascale.serror{at}jouy.inra.fr.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, October 2006, p. 6858-6868, Vol. 188, No. 19
0021-9193/06/$08.00+0     doi:10.1128/JB.00421-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Gaspar, F., Teixeira, N., Rigottier-Gois, L., Marujo, P., Nielsen-LeRoux, C., Crespo, M. T. B., Lopes, M. d. F. S., Serror, P. (2009). Virulence of Enterococcus faecalis dairy strains in an insect model: the role of fsrB and gelE. Microbiology 155: 3564-3571 [Abstract] [Full Text]  
  • Forquin, M.-P., Duvergey, H., Proux, C., Loux, V., Mounier, J., Landaud, S., Coppee, J.-Y., Gibrat, J.-F., Bonnarme, P., Martin-Verstraete, I., Vallaeys, T. (2009). Identification of Brevibacteriaceae by Multilocus Sequence Typing and Comparative Genomic Hybridization Analyses. Appl. Environ. Microbiol. 75: 6406-6409 [Abstract] [Full Text]  
  • McBride, S. M., Coburn, P. S., Baghdayan, A. S., Willems, R. J. L., Grande, M. J., Shankar, N., Gilmore, M. S. (2009). Genetic Variation and Evolution of the Pathogenicity Island of Enterococcus faecalis. J. Bacteriol. 191: 3392-3402 [Abstract] [Full Text]  
  • Mesnage, S., Chau, F., Dubost, L., Arthur, M. (2008). Role of N-Acetylglucosaminidase and N-Acetylmuramidase Activities in Enterococcus faecalis Peptidoglycan Metabolism. J. Biol. Chem. 283: 19845-19853 [Abstract] [Full Text]  
  • Hendrickx, A. P. A., van Wamel, W. J. B., Posthuma, G., Bonten, M. J. M., Willems, R. J. L. (2007). Five Genes Encoding Surface-Exposed LPXTG Proteins Are Enriched in Hospital-Adapted Enterococcus faecium Clonal Complex 17 Isolates. J. Bacteriol. 189: 8321-8332 [Abstract] [Full Text]  
  • Macovei, L., Zurek, L. (2007). Influx of Enterococci and Associated Antibiotic Resistance and Virulence Genes from Ready-To-Eat Food to the Human Digestive Tract. Appl. Environ. Microbiol. 73: 6740-6747 [Abstract] [Full Text]  
  • Aakra, A., Nyquist, O. L., Snipen, L., Reiersen, T. S., Nes, I. F. (2007). Survey of Genomic Diversity among Enterococcus faecalis Strains by Microarray-Based Comparative Genomic Hybridization. Appl. Environ. Microbiol. 73: 2207-2217 [Abstract] [Full Text]  
  • Brinster, S., Furlan, S., Serror, P. (2007). C-Terminal WxL Domain Mediates Cell Wall Binding in Enterococcus faecalis and Other Gram-Positive Bacteria. J. Bacteriol. 189: 1244-1253 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»