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Journal of Bacteriology, January 2006, p. 724-732, Vol. 188, No. 2
0021-9193/06/$08.00+0     doi:10.1128/JB.188.2.724-732.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Two-Component Systems of Corynebacterium glutamicum: Deletion Analysis and Involvement of the PhoS-PhoR System in the Phosphate Starvation Response§

Martina Kocan,{dagger} Steffen Schaffer,{dagger},{ddagger} Takeru Ishige, Ulrike Sorger-Herrmann, Volker F. Wendisch, and Michael Bott*

Institut für Biotechnologie 1, Forschungszentrum Jülich, D-52425 Jülich, Germany

Received 15 July 2005/ Accepted 22 October 2005

Corynebacterium glutamicum contains genes for 13 two-component signal transduction systems. In order to test for their essentiality and involvement in the adaptive response to phosphate (Pi) starvation, a set of 12 deletion mutants was constructed. One of the mutants was specifically impaired in its ability to grow under Pi limitation, and therefore the genes lacking in this strain were named phoS (encoding the sensor kinase) and phoR (encoding the response regulator). DNA microarray analyses with the C. glutamicum wild type and the {Delta}phoRS mutant supported a role for the PhoRS system in the adaptation to Pi starvation. In contrast to the wild type, the {Delta}phoRS mutant did not induce the known Pi starvation-inducible (psi) genes within 1 hour after a shift from Pi excess to Pi limitation, except for the pstSCAB operon, which was still partially induced. This indicates an activator function for PhoR and the existence of at least one additional regulator of the pst operon. Primer extension analysis of selected psi genes (pstS, ugpA, phoR, ushA, and nucH) confirmed the microarray data and provided evidence for positive autoregulation of the phoRS genes.

* Corresponding author. Mailing address: Institut für Biotechnologie 1, Forschungszentrum Jülich, D-52425 Jülich, Germany. Phone: 49 2461 61 5515. Fax: 49 2461 61 2710. E-mail: m.bott{at}fz-juelich.de.

§ Supplemental material for this article may be found at http://jb.asm.org/.

{dagger} These authors contributed equally to this work.

{ddagger} Present address: Project House ProFerm, Degussa AG, D-63457 Hanau, Germany.

Journal of Bacteriology, January 2006, p. 724-732, Vol. 188, No. 2
0021-9193/06/$08.00+0     doi:10.1128/JB.188.2.724-732.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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