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Journal of Bacteriology, January 2006, p. 784-788, Vol. 188, No. 2
0021-9193/06/$08.00+0     doi:10.1128/JB.188.2.784-788.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Quorum-Sensing Signal Synthesis by the Yersinia pestis Acyl-Homoserine Lactone Synthase YspI

J. Paul Kirwan,1,2 Ty A. Gould,1 Herbert P. Schweizer,3 Scott W. Bearden,4 Robert C. Murphy,1 and Mair E. A. Churchill1*

Department of Pharmacology, Program in Biomolecular Structure, The University of Colorado Health Sciences Center, P.O. Box 8511 MS8303, Aurora, Colorado 80045,1 Department of Chemistry, University of Colorado at Denver, 1200 Larimer Street, Denver, Colorado 80217,2 Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523,3 Centers for Disease Control and Prevention, Division of Vector-Borne Infectious Diseases, P.O. Box 2087, Fort Collins, Colorado 805224

Received 7 July 2005/ Accepted 22 October 2005

The acyl-homoserine lactone molecular species (AHLs) produced by the Yersinia pestis AHL synthase YspI were identified by biochemical and physical/chemical techniques. Bioassays of extracts from culture supernatants of the recombinant YspI and wild-type Yersinia pestis showed similar profiles of AHLs. Analysis by liquid chromatography-mass spectrometry revealed that the predominant AHLs were N-3-oxooctanoyl-L-homoserine lactone and N-3-oxo-hexanoyl-L-homoserine lactone.

* Corresponding author. Mailing address: Department of Pharmacology, Program in Biomolecular Structure, The University of Colorado Health Sciences Center, P.O. Box 8511 MS8303, Aurora, CO 80045. Phone: (303) 724-3670. Fax: (303) 724-3663. E-mail: mair.churchill{at}uchsc.edu.

Journal of Bacteriology, January 2006, p. 784-788, Vol. 188, No. 2
0021-9193/06/$08.00+0     doi:10.1128/JB.188.2.784-788.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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  • Khajanchi, B. K., Sha, J., Kozlova, E. V., Erova, T. E., Suarez, G., Sierra, J. C., Popov, V. L., Horneman, A. J., Chopra, A. K. (2009). N-Acylhomoserine lactones involved in quorum sensing control the type VI secretion system, biofilm formation, protease production, and in vivo virulence in a clinical isolate of Aeromonas hydrophila. Microbiology 155: 3518-3531 [Abstract] [Full Text]  
  • Gould, T. A., Herman, J., Krank, J., Murphy, R. C., Churchill, M. E. A. (2006). Specificity of Acyl-Homoserine Lactone Synthases Examined by Mass Spectrometry. J. Bacteriol. 188: 773-783 [Abstract] [Full Text]  


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