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Journal of Bacteriology, January 2006, p. 805-808, Vol. 188, No. 2
0021-9193/06/$08.00+0     doi:10.1128/JB.188.2.805-808.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Intact Carboxysomes in a Cyanobacterial Cell Visualized by Hilbert Differential Contrast Transmission Electron Microscopy

Yasuko Kaneko,1 Radostin Danev,2 Kuniaki Nagayama,2 and Hitoshi Nakamoto3*

Department of Regulation Biology, Saitama University, Saitama, 338-8570, Japan,1 Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, Myodaiji-cho, Okazaki 444-8787, Japan,2 Department of Biochemistry and Molecular Biology, Saitama University, Saitama, 338-8570, Japan3

Received 23 June 2005/ Accepted 27 October 2005

Carboxysomes in rapidly frozen ice-embedded whole cells of the cyanobacterium Synechococcus sp. strain PCC 7942 were visualized by the recently developed Hilbert differential contrast transmission electron microscope. Structural details of carboxysomes were especially clearly visualized in the ruptured cells. The novel electron microscopy exhibited the paracrystalline arrays of molecules of the enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase in the carboxysomes in much better contrast than conventional transmission electron microscopy with ultrathin sections of cells. The carboxysome was surrounded by a 5- to 6-nm-thick monolayer shell which consisted of orderly arrays of globular particles.

* Corresponding author. Mailing address: Department of Biochemistry and Molecular Biology, Saitama University, 255 Shimo-Okubo, Sakura-ku, Saitama 338-8570, Japan. Phone: 81-48-858-3403. Fax: 81-48-858-3384. E-mail: nakamoto{at}post.saitama-u.ac.jp.

Journal of Bacteriology, January 2006, p. 805-808, Vol. 188, No. 2
0021-9193/06/$08.00+0     doi:10.1128/JB.188.2.805-808.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Atsuzawa, K., Usuda, N., Nakazawa, A., Fukasawa, M., Danev, R., Sugitani, S., Nagayama, K. (2009). High-contrast imaging of plastic-embedded tissues by phase contrast electron microscopy. J Electron Microsc (Tokyo) 58: 35-45 [Abstract] [Full Text]  
  • Nagayama, K., Danev, R. (2008). Phase contrast electron microscopy: development of thin-film phase plates and biological applications. Phil Trans R Soc B 363: 2153-2162 [Abstract] [Full Text]  


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