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Journal of Bacteriology, October 2006, p. 7039-7048, Vol. 188, No. 20
0021-9193/06/$08.00+0     doi:10.1128/JB.00552-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

The Three-Dimensional Structure of the Flagellar Rotor from a Clockwise-Locked Mutant of Salmonella enterica Serovar Typhimurium

Dennis R. Thomas,1,3 Noreen R. Francis,1 Chen Xu,1 and David J. DeRosier1,2*

Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, Massachusetts 02454,1 Department of Biology, Brandeis University, Waltham, Massachusetts 02454,2 Howard Hughes Medical Institute, Brandeis University, Waltham, Massachusetts 024543

Received 18 April 2006/ Accepted 17 July 2006

Three-dimensional reconstructions from electron cryomicrographs of the rotor of the flagellar motor reveal that the symmetry of individual M rings varies from 24-fold to 26-fold while that of the C rings, containing the two motor/switch proteins FliM and FliN, varies from 32-fold to 36-fold, with no apparent correlation between the symmetries of the two rings. Results from other studies provided evidence that, in addition to the transmembrane protein FliF, at least some part of the third motor/switch protein, FliG, contributes to a thickening on the face of the M ring, but there was no evidence as to whether or not any portion of FliG also contributes to the C ring. Of the four morphological features in the cross section of the C ring, the feature closest to the M ring is not present with the rotational symmetry of the rest of the C ring, but instead it has the symmetry of the M ring. We suggest that this inner feature arises from a domain of FliG. We present a hypothetical docking in which the C-terminal motor domain of FliG lies in the C ring, where it can interact intimately with FliM.

* Corresponding author. Mailing address: Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, MA 02454. Phone: (781) 736-2494. Fax: (781) 736-2419. E-mail: derosier{at}brandeis.edu.

Journal of Bacteriology, October 2006, p. 7039-7048, Vol. 188, No. 20
0021-9193/06/$08.00+0     doi:10.1128/JB.00552-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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