This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Weber, A.
Right arrow Articles by Jung, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Weber, A.
Right arrow Articles by Jung, K.

 Previous Article  |  Next Article 

Journal of Bacteriology, October 2006, p. 7165-7175, Vol. 188, No. 20
0021-9193/06/$08.00+0     doi:10.1128/JB.00508-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Time-Dependent Proteome Alterations under Osmotic Stress during Aerobic and Anaerobic Growth in Escherichia coli

Arnim Weber,{dagger} Stephanie A. Kögl,{dagger} and Kirsten Jung*

Ludwig-Maximilians-Universität, Department Biologie I, Bereich Mikrobiologie, D-80638 München, Germany

Received 10 April 2006/ Accepted 18 July 2006

Escherichia coli lives in the mammalian gastrointestinal tract anaerobically at high osmolarity as well as in the soil aerobically at varying osmolarities. Adaptation to these varying environmental conditions is crucial for growth and survival of E. coli. Two-dimensional protein gels were used to visualize global time-dependent changes (10 to 60 min) in the proteome of cells responding to osmotic stress (0.4 M NaCl or 0.7 M sorbitol) under aerobic or anaerobic conditions. The protein profiles revealed an induction of 12 proteins (Dps, HchA, HdhA, InfB, OsmC, OsmY, ProX, KatE, PspA, TalA, TktB, and TreF) under osmotic stress in an aerobic milieu. Eleven additional proteins (OtsB, YceI, YciE, YciF, YgaU, YjbJ, AcnA, MetL, PoxB, Ssb, and YhbO) were induced by osmotic stress imposed by NaCl. Most of the accumulated proteins were cross-protecting proteins (e.g., OsmY, OsmC, Dps, and KatE) which are regulated at the transcriptional level predominantly by RpoS and other regulators (e.g., integration host factor, OxyR, H-NS, LRP, and FIS). Comparative analysis of the proteome of E. coli grown under aerobic or anaerobic conditions under osmotic stress (NaCl) revealed an overlap of the up-regulated proteins of more than 50%. Ten proteins (PoxB, AcnA, TalA, TktB, KatE, PspA, Ssb, TreF, MetL, and YhbO) were detectable only under aerobic, high-osmolality conditions. Time-dependent alterations of the proteome were monitored, allowing classification of the up-regulated proteins into early, middle, and long-term phases of adaptation. Only a few proteins were found to be down-regulated upon osmotic stress.

* Corresponding author. Mailing address: Ludwig-Maximilians-Universität München, Department Biologie I, Bereich Mikrobiologie, Maria-Ward-Str. 1a, D-80638 München, Germany. Phone: 49-89-2180-6120. Fax: 49-89-2180-6122. E-mail: kirsten.jung{at}lrz.uni-muenchen.de.

{dagger} These authors contributed equally to this work.

Journal of Bacteriology, October 2006, p. 7165-7175, Vol. 188, No. 20
0021-9193/06/$08.00+0     doi:10.1128/JB.00508-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Martinez-Salazar, J. M., Salazar, E., Encarnacion, S., Ramirez-Romero, M. A., Rivera, J. (2009). Role of the Extracytoplasmic Function Sigma Factor RpoE4 in Oxidative and Osmotic Stress Responses in Rhizobium etli. J. Bacteriol. 191: 4122-4132 [Abstract] [Full Text]  
  • Encheva, V., Shah, H. N., Gharbia, S. E. (2009). Proteomic analysis of the adaptive response of Salmonella enterica serovar Typhimurium to growth under anaerobic conditions. Microbiology 155: 2429-2441 [Abstract] [Full Text]  
  • Golovina, A. Y., Sergiev, P. V., Golovin, A. V., Serebryakova, M. V., Demina, I., Govorun, V. M., Dontsova, O. A. (2009). The yfiC gene of E. coli encodes an adenine-N6 methyltransferase that specifically modifies A37 of tRNA1Val(cmo5UAC). RNA 15: 1134-1141 [Abstract] [Full Text]  
  • Bergholz, T. M., Vanaja, S. K., Whittam, T. S. (2009). Gene Expression Induced in Escherichia coli O157:H7 upon Exposure to Model Apple Juice. Appl. Environ. Microbiol. 75: 3542-3553 [Abstract] [Full Text]  
  • Barth, E., Gora, K. V., Gebendorfer, K. M., Settele, F., Jakob, U., Winter, J. (2009). Interplay of cellular cAMP levels, {sigma}S activity and oxidative stress resistance in Escherichia coli. Microbiology 155: 1680-1689 [Abstract] [Full Text]  
  • Dutta, D., Bandyopadhyay, K., Datta, A. B., Sardesai, A. A., Parrack, P. (2009). Properties of HflX, an Enigmatic Protein from Escherichia coli. J. Bacteriol. 191: 2307-2314 [Abstract] [Full Text]  
  • Salvetti, S., Celandroni, F., Ceragioli, M., Senesi, S., Ghelardi, E. (2009). Identification of non-flagellar genes involved in swarm cell differentiation using a Bacillus thuringiensis mini-Tn10 mutant library. Microbiology 155: 912-921 [Abstract] [Full Text]  
  • Barreiro, C., Nakunst, D., Huser, A. T., de Paz, H. D., Kalinowski, J., Martin, J. F. (2009). Microarray studies reveal a 'differential response' to moderate or severe heat shock of the HrcA- and HspR-dependent systems in Corynebacterium glutamicum. Microbiology 155: 359-372 [Abstract] [Full Text]  
  • Amato, P., Christner, B. C. (2009). Energy Metabolism Response to Low-Temperature and Frozen Conditions in Psychrobacter cryohalolentis. Appl. Environ. Microbiol. 75: 711-718 [Abstract] [Full Text]  
  • Price, C. E., Driessen, A. J. M. (2008). YidC Is Involved in the Biogenesis of Anaerobic Respiratory Complexes in the Inner Membrane of Escherichia coli. J. Biol. Chem. 283: 26921-26927 [Abstract] [Full Text]  
  • Gunasekera, T. S., Csonka, L. N., Paliy, O. (2008). Genome-Wide Transcriptional Responses of Escherichia coli K-12 to Continuous Osmotic and Heat Stresses. J. Bacteriol. 190: 3712-3720 [Abstract] [Full Text]  
  • Chopra, S., Dooling, R. M., Horner, C. G., Howell, E. E. (2008). A Balancing Act between Net Uptake of Water during Dihydrofolate Binding and Net Release of Water upon NADPH Binding in R67 Dihydrofolate Reductase. J. Biol. Chem. 283: 4690-4698 [Abstract] [Full Text]  
  • Abdallah, J., Caldas, T., Kthiri, F., Kern, R., Richarme, G. (2007). YhbO Protects Cells against Multiple Stresses. J. Bacteriol. 189: 9140-9144 [Abstract] [Full Text]  
  • Huang, Y., Leming, C. L., Suyemoto, M., Altier, C. (2007). Genome-Wide Screen of Salmonella Genes Expressed during Infection in Pigs, Using In Vivo Expression Technology. Appl. Environ. Microbiol. 73: 7522-7530 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»