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Journal of Bacteriology, October 2006, p. 7317-7320, Vol. 188, No. 20
0021-9193/06/$08.00+0     doi:10.1128/JB.00383-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Evidence for Conformational Changes within DsbD: Possible Role for Membrane-Embedded Proline Residues

Annie Hiniker,1,2 Didier Vertommen,3 James C. A. Bardwell,1,2 and Jean-Francois Collet4*

Program in Cellular and Molecular Biology, Department of Molecular, Cellular and Developmental Biology,1 Medical Scientist Training Program, University of Michigan, Ann Arbor, Michigan 48109-1048,2 Unit Hormones and Metabolism,3 Laboratory of Physiological Chemistry, Christian de Duve Institute of Cellular Pathology, Université Catholique de Louvain, B-1200 Brussels, Belgium4

Received 17 March 2006/ Accepted 21 July 2006

The mechanism by which DsbD transports electrons across the cytoplasmic membrane is unknown. Here we provide evidence that DsbD's conformation depends on its oxidation state. Our data also suggest that four highly conserved prolines surrounding DsbD's membrane-embedded catalytic cysteines may have an important functional role, possibly conferring conformational flexibility to DsbD.

* Corresponding author. Mailing address: BCHM-GRM, Université Catholique de Louvain, ICP, 75-39 Avenue Hippocrate, B-1200 Brussels, Belgium. Phone: 32-2-764-7565. Fax: 32-2-764-7598. E-mail: collet{at}bchm.ucl.ac.be.

Journal of Bacteriology, October 2006, p. 7317-7320, Vol. 188, No. 20
0021-9193/06/$08.00+0     doi:10.1128/JB.00383-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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