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Journal of Bacteriology, November 2006, p. 7512-7520, Vol. 188, No. 21
0021-9193/06/$08.00+0     doi:10.1128/JB.00980-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Novel Role of mfd: Effects on Stationary-Phase Mutagenesis in Bacillus subtilis{triangledown}

Christian Ross,1 Christine Pybus,1 Mario Pedraza-Reyes,2 Huang-Mo Sung,1 Ronald E. Yasbin,1 and Eduardo Robleto1*

Department of Biological Sciences, University of Nevada, Las Vegas, Las Vegas, Nevada 89154-4004,1 Institute of Investigation in Experimental Biology, Faculty of Chemistry, University of Guanajuato, Guanajuato, Mexico 360502

Received 5 July 2006/ Accepted 22 August 2006

Previously, using a chromosomal reversion assay system, we established that an adaptive mutagenic process occurs in nongrowing Bacillus subtilis cells under stress, and we demonstrated that multiple mechanisms are involved in generating these mutations (41, 43). In an attempt to delineate how these mutations are generated, we began an investigation into whether or not transcription and transcription-associated proteins influence adaptive mutagenesis. In B. subtilis, the Mfd protein (transcription repair coupling factor) facilitates removal of RNA polymerase stalled at transcriptional blockages and recruitment of repair proteins to DNA lesions on the transcribed strand. Here we demonstrate that the loss of Mfd has a depressive effect on stationary-phase mutagenesis. An association between Mfd mutagenesis and aspects of transcription is discussed.

* Corresponding author. Mailing address: Department of Biological Sciences, University of Nevada, Las Vegas, Las Vegas, NV 89154-4004. Phone: (702) 895-2496. Fax: (702) 895-3956. E-mail: eduardo.robleto{at}unlv.edu.

{triangledown} Published ahead of print on 1 September 2006.

Journal of Bacteriology, November 2006, p. 7512-7520, Vol. 188, No. 21
0021-9193/06/$08.00+0     doi:10.1128/JB.00980-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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  • Vidales, L. E., Cardenas, L. C., Robleto, E., Yasbin, R. E., Pedraza-Reyes, M. (2009). Defects in the Error Prevention Oxidized Guanine System Potentiate Stationary-Phase Mutagenesis in Bacillus subtilis. J. Bacteriol. 191: 506-513 [Abstract] [Full Text]  
  • Hasegawa, K., Yoshiyama, K., Maki, H. (2008). Spontaneous mutagenesis associated with nucleotide excision repair in Escherichia coli.. GENES CELLS 13: 459-469 [Abstract] [Full Text]  


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