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Journal of Bacteriology, November 2006, p. 7617-7625, Vol. 188, No. 21
0021-9193/06/$08.00+0     doi:10.1128/JB.00719-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Transcriptome Profiling Reveals the Importance of Plasmid pSymB for Osmoadaptation of Sinorhizobium meliloti{triangledown} ,{dagger}

Ana Domínguez-Ferreras,1 Rebeca Pérez-Arnedo,1 Anke Becker,2 José Olivares,1 María J. Soto,1 and Juan Sanjuán1*

Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, CSIC, Granada, Spain,1 Lehrstuhl für Genetik, Fakultät für Biologie, Universität Bielefeld, D-33501 Bielefeld, Germany2

Received 19 May 2006/ Accepted 7 August 2006

In this work, DNA microarrays were used to investigate genome-wide transcriptional responses of Sinorhizobium meliloti to a sudden increase in external osmolarity elicited by addition of either NaCl or sucrose to exponentially growing cultures. A time course of the response within the first 4 h after the osmotic shock was established. We found that there was a general redundancy in the differentially expressed genes after NaCl or sucrose addition. Both kinds of stress resulted in induction of a large number of genes having unknown functions and in repression of many genes coding for proteins with known functions. There was a strong replicon bias in the pattern of the osmotic stress response; whereas 64% of the upregulated genes had a plasmid localization, 85% of the downregulated genes were chromosomal. Among the pSymB osmoresponsive genes, 83% were upregulated, suggesting the importance of this plasmid for S. meliloti osmoadaptation. Indeed, we identified a 200-kb region in pSymB needed for adaptation to saline shock which has a high density of osmoregulated genes.

* Corresponding author. Mailing address: Estación Experimental del Zaidin, CSIC, Prof. Albareda 1, E-18008 Granada, Spain. Phone: 34-958-181600, ext. 259. Fax: 34-958-129600. E-mail: Juan.Sanjuan{at}eez.csic.es.

{triangledown} Published ahead of print on 17 August 2006.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, November 2006, p. 7617-7625, Vol. 188, No. 21
0021-9193/06/$08.00+0     doi:10.1128/JB.00719-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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