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Journal of Bacteriology, November 2006, p. 7985-7987, Vol. 188, No. 22
0021-9193/06/$08.00+0     doi:10.1128/JB.00886-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Mutation of His-157 in the Second Pore Loop Drastically Reduces the Activity of the Synechocystis Ktr-Type Transporter{triangledown}

Lalu Zulkifli and Nobuyuki Uozumi*

Bioscience and Biotechnology Center, Nagoya University, Nagoya 464-8601, Japan

Received 20 June 2006/ Accepted 4 September 2006

Mutation of a conserved His-157 in the second pore loop of KtrB drastically reduced the activity of the K+ transporter from Synechocystis sp. strain PCC 6803. This result suggests that His-157 plays an essential role in the K+ transport activity of the transporter system.

* Corresponding author. Mailing address: Bioscience and Biotechnology Center, Nagoya University, Nagoya 464-8601, Japan. Phone: 81-52-789-5202. Fax: 81-52-789-5206. E-mail: uozumi{at}agr.nagoya-u.ac.jp.

{triangledown} Published ahead of print on 15 September 2006.

Journal of Bacteriology, November 2006, p. 7985-7987, Vol. 188, No. 22
0021-9193/06/$08.00+0     doi:10.1128/JB.00886-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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