This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Krings, E.
Right arrow Articles by Eggeling, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Krings, E.
Right arrow Articles by Eggeling, L.

 Previous Article  |  Next Article 

Journal of Bacteriology, December 2006, p. 8054-8061, Vol. 188, No. 23
0021-9193/06/$08.00+0     doi:10.1128/JB.00935-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Characterization of myo-Inositol Utilization by Corynebacterium glutamicum: the Stimulon, Identification of Transporters, and Influence on L-Lysine Formation{triangledown}

Eva Krings,1 Karin Krumbach,1 Brigitte Bathe,2 Ralf Kelle,2 Volker F. Wendisch,3 Hermann Sahm,1 and Lothar Eggeling1*

Institute of Biotechnology, Research Centre Juelich, D-52425 Juelich, Germany,1 Degussa R&D Feed Additives/Biotechnology, P.O. Box 1112, D-33788 Halle, Germany,2 Institute of Molecular Microbiology and Biotechnology, University of Münster, Corrensstrasse 3, D-48149 Münster, Germany3

Received 28 June 2006/ Accepted 18 September 2006

Although numerous bacteria possess genes annotated iol in their genomes, there have been very few studies on the possibly associated myo-inositol metabolism and its significance for the cell. We found that Corynebacterium glutamicum utilizes myo-inositol as a carbon and energy source, enabling proliferation with a high maximum rate of 0.35 h–1. Whole-genome DNA microarray analysis revealed that 31 genes respond to myo-inositol utilization, with 21 of them being localized in two clusters of >14 kb. A set of genomic mutations and functional studies yielded the result that some genes in the two clusters are redundant, and only cluster I is necessary for catabolizing the polyol. There are three genes which encode carriers belonging to the major facilitator superfamily and which exhibit a >12-fold increased mRNA level on myo-inositol. As revealed by mutant characterizations, one carrier is not involved in myo-inositol uptake whereas the other two are active and can completely replace each other with apparent Kms for myo-inositol as a substrate of 0.20 mM and 0.45 mM, respectively. Interestingly, upon utilization of myo-inositol, the L-lysine yield is 0.10 mol/mol, as opposed to 0.30 mol/mol, with glucose as the substrate. This is probably not only due to myo-inositol metabolism alone since a mixture of 187 mM glucose and 17 mM myo-inositol, where the polyol only contributes 8% of the total carbon, reduced the L-lysine yield by 29%. Moreover, genome comparisons with other bacteria highlight the core genes required for growth on myo-inositol, whose metabolism is still weakly defined.

* Corresponding author. Mailing address: Institute of Biotechnology, Research Centre Juelich, D-52425 Juelich, Germany. Phone: 0049 2461 615132. Fax: 00492461612710. E-mail: l.eggeling{at}fz-juelich.de.

{triangledown} Published ahead of print on 22 September 2006.

Journal of Bacteriology, December 2006, p. 8054-8061, Vol. 188, No. 23
0021-9193/06/$08.00+0     doi:10.1128/JB.00935-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Reynolds, T. B. (2009). Strategies for acquiring the phospholipid metabolite inositol in pathogenic bacteria, fungi and protozoa: making it and taking it. Microbiology 155: 1386-1396 [Abstract] [Full Text]  
  • Kroger, C., Fuchs, T. M. (2009). Characterization of the myo-Inositol Utilization Island of Salmonella enterica serovar Typhimurium. J. Bacteriol. 191: 545-554 [Abstract] [Full Text]  
  • Rittmann, D., Lindner, S. N., Wendisch, V. F. (2008). Engineering of a Glycerol Utilization Pathway for Amino Acid Production by Corynebacterium glutamicum. Appl. Environ. Microbiol. 74: 6216-6222 [Abstract] [Full Text]  
  • Yoshida, K.-i., Yamaguchi, M., Morinaga, T., Kinehara, M., Ikeuchi, M., Ashida, H., Fujita, Y. (2008). myo-Inositol Catabolism in Bacillus subtilis. J. Biol. Chem. 283: 10415-10424 [Abstract] [Full Text]  
  • Dordet-Frisoni, E., Dorchies, G., De Araujo, C., Talon, R., Leroy, S. (2007). Genomic Diversity in Staphylococcus xylosus. Appl. Environ. Microbiol. 73: 7199-7209 [Abstract] [Full Text]  
  • Yebra, M. J., Zuniga, M., Beaufils, S., Perez-Martinez, G., Deutscher, J., Monedero, V. (2007). Identification of a Gene Cluster Enabling Lactobacillus casei BL23 To Utilize myo-Inositol. Appl. Environ. Microbiol. 73: 3850-3858 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»