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Journal of Bacteriology, December 2006, p. 8109-8117, Vol. 188, No. 23
0021-9193/06/$08.00+0     doi:10.1128/JB.01262-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

The ColRS Two-Component System Regulates Membrane Functions and Protects Pseudomonas putida against Phenol{triangledown}

Paula Ann Kivistik, Marta Putrins, Külliki Püvi, Heili Ilves, Maia Kivisaar, and Rita Hõrak*

Estonian Biocentre and Institute of Molecular and Cell Biology, Tartu University, 51010 Tartu, Estonia

Received 10 August 2006/ Accepted 18 September 2006

As reported, the two-component system ColRS is involved in two completely different processes. It facilitates the root colonization ability of Pseudomonas fluorescens and is necessary for the Tn4652 transposition-dependent accumulation of phenol-utilizing mutants in Pseudomonas putida. To determine the role of the ColRS system in P. putida, we searched for target genes of response regulator ColR by use of a promoter library. Promoter screening was performed on phenol plates to mimic the conditions under which the effect of ColR on transposition was detected. The library screen revealed the porin-encoding gene oprQ and the alginate biosynthesis gene algD occurring under negative control of ColR. Binding of ColR to the promoter regions of oprQ and algD in vitro confirmed its direct involvement in regulation of these genes. Additionally, the porin-encoding gene ompAPP0773 and the type I pilus gene csuB were also identified in the promoter screen. However, it turned out that ompAPP0773 and csuB were actually affected by phenol and that the influence of ColR on these promoters was indirect. Namely, our results show that ColR is involved in phenol tolerance of P. putida. Phenol MIC measurement demonstrated that a colR mutant strain did not tolerate elevated phenol concentrations. Our data suggest that increased phenol susceptibility is also the reason for inhibition of transposition of Tn4652 in phenol-starving colR mutant bacteria. Thus, the current study revealed the role of the ColRS two-component system in regulation of membrane functionality, particularly in phenol tolerance of P. putida.

* Corresponding author. Mailing address: Estonian Biocentre and Institute of Molecular and Cell Biology, Tartu University, 23 Riia Street, 51010 Tartu, Estonia. Phone: 372-7-375015. Fax: 372-7-420286. E-mail: rhorak{at}ebc.ee.

{triangledown} Published ahead of print on 29 September 2006.

Journal of Bacteriology, December 2006, p. 8109-8117, Vol. 188, No. 23
0021-9193/06/$08.00+0     doi:10.1128/JB.01262-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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