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Journal of Bacteriology, December 2006, p. 8145-8152, Vol. 188, No. 23
0021-9193/06/$08.00+0     doi:10.1128/JB.01011-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Surface Protein IsdC and Sortase B Are Required for Heme-Iron Scavenging of Bacillus anthracis{triangledown}

Anthony W. Maresso, Travis J. Chapa, and Olaf Schneewind*

Department of Microbiology, University of Chicago, 920 East 58th Street, Chicago, Illinois 60637

Received 11 July 2006/ Accepted 15 September 2006

Bacillus anthracis, the spore-forming agent of anthrax, requires iron for growth and is capable of scavenging heme-iron during infection. We show here that the B. anthracis iron-regulated surface determinants (isd) locus encompasses isdC, specifying a heme-iron binding surface protein. Anchoring of IsdC to the cell wall envelopes of vegetative bacilli requires srtB, which encodes sortase B. Purified sortase B cleaves IsdC between the threonine and the glycine of its NPKTG motif sorting signal. B. anthracis variants lacking either isdC or srtB display defects in heme-iron scavenging, suggesting that IsdC binding to heme-iron in the cell wall envelope contributes to bacterial uptake of heme.

* Corresponding author. Mailing address: Department of Microbiology, University of Chicago, CLSC 607b, 920 E 58th Street, Chicago, IL 60637. Phone: (773) 834-9060. Fax: (773) 834-8150. E-mail: oschnee{at}bsd.uchicago.edu.

{triangledown} Published ahead of print on 29 September 2006.

Journal of Bacteriology, December 2006, p. 8145-8152, Vol. 188, No. 23
0021-9193/06/$08.00+0     doi:10.1128/JB.01011-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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