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Journal of Bacteriology, December 2006, p. 8317-8320, Vol. 188, No. 23
0021-9193/06/$08.00+0     doi:10.1128/JB.00977-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

The Escherichia coli rpoS-Dependent htrC Gene Is Not Involved in the Heat Shock Response{triangledown}

Zubin Thacker,1 Elise Darmon,1 France Keppel,2 and Millicent Masters1*

University of Edinburgh, School of Biology, King's Buildings, Mayfield Road, Edinburgh EH9 3JR, Scotland,1 Département de microbiologie et médecine moléculaire, Centre Médical Universitaire, 1 rue Michel Servet, 1211 Geneve 4, Suisse2

Received 5 July 2006/ Accepted 7 September 2006

We found that a new mutant with a deletion/replacement of the Escherichia coli K-12 htrC gene, a gene previously reported to be required for growth at elevated temperatures, is not temperature sensitive. Furthermore, the original mutants, kindly provided by the original authors, although temperature sensitive, do not have mutations in the open reading frame designated htrC. We found that htrC requires RpoS for enhanced expression in the early stationary phase and is expressed at very low levels until then. The growth of our htrC mutant slowed during the early stationary phase, and the mutant was replaced by its parent in mixed cultures. Since we cannot assign a function or distinctive phenotype to htrC, we suggest that this open reading frame should be given a positional designation, yjaZ, until a specific function is identified.

* Corresponding author. Mailing address: University of Edinburgh, School of Biology, Darwin Building, King's Building, Mayfield Road, Edinburgh EH9 3JR, Scotland. Phone: 44 (0)131 650 5355. Fax: 44 (0)131 650 8650. E-mail: M.Masters{at}ed.ac.uk.

{triangledown} Published ahead of print on 15 September 2006.

Journal of Bacteriology, December 2006, p. 8317-8320, Vol. 188, No. 23
0021-9193/06/$08.00+0     doi:10.1128/JB.00977-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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