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Journal of Bacteriology, February 2006, p. 1205-1210, Vol. 188, No. 3
0021-9193/06/$08.00+0     doi:10.1128/JB.188.3.1205-1210.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

The Vibrio harveyi GTPase CgtA_V Is Essential and Is Associated with the 50S Ribosomal Subunit

A. E. Sikora,^1,2 R. Zielke,^1,2 K. Datta,¹ and J. R. Maddock^1*

Department of Molecular, Cellular and Developmental Biology, University of Michigan, 830 North University, Ann Arbor, Michigan 48109,¹ Department of Molecular Biology, University of Gdansk, Kadki 24, 80-822 Gdansk, Poland²

Received 14 September 2005/ Accepted 28 October 2005

It was previously reported that unlike the other obg/cgtA GTPases, the Vibrio harveyi cgtA_V is not essential. Here we show that cgtA_V was not disrupted in these studies and is, in fact, essential for viability. Depletion of CgtA_V did not result in cell elongation. CgtA_V is associated with the large ribosomal particle. In light of our results, we predict that the V. harveyi CgtA_V protein plays a similar essential role to that seen for Obg/CgtA proteins in other bacteria.

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* Corresponding author. Mailing address: Department of Molecular, Cellular and Developmental Biology, University of Michigan, 830 North University, Ann Arbor, MI 48109-1048. Phone: (734) 936-8068. Fax: (734) 647-0884. E-mail: maddock{at}umich.edu.

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Journal of Bacteriology, February 2006, p. 1205-1210, Vol. 188, No. 3
0021-9193/06/$08.00+0     doi:10.1128/JB.188.3.1205-1210.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

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