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Journal of Bacteriology, February 2006, p. 977-987, Vol. 188, No. 3
0021-9193/06/$08.00+0     doi:10.1128/JB.188.3.977-987.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

A selC-Associated Genomic Island of the Extraintestinal Avian Pathogenic Escherichia coli Strain BEN2908 Is Involved in Carbohydrate Uptake and Virulence

Iman Chouikha, Pierre Germon, Annie Brée, Philippe Gilot, Maryvonne Moulin-Schouleur, and Catherine Schouler*

Equipe de Pathologie Bactérienne, UR86, INRA, 37380 Nouzilly, France

Received 11 August 2005/ Accepted 28 October 2005

The complete nucleotide sequence and genetic organization of a new genomic island (AGI-3) isolated from the extraintestinal avian pathogenic Escherichia coli strain BEN2908 is reported. This 49,600-bp island is inserted at the selC locus and contains putative mobile genetic elements such as a phage-related integrase gene, transposase genes, and direct repeats. AGI-3 shows a mosaic structure of five modules. Some of these modules are present in other E. coli strains and in other pathogenic bacterial species. The gene cluster aec-35 to aec-37 of module 1 encodes proteins associated with carbohydrates assimilation such as a major facilitator superfamily transporter (Aec-36), a glycosidase (Aec-37), and a putative transcriptional regulator of the LacI family (Aec-35). The aec-35 to aec-37 cluster was found in 11.6% of the tested pathogenic and nonpathogenic E. coli strains. When present, the aec-35 to aec-37 cluster is strongly associated with the selC locus (97%). Deletion of the aec-35-aec-37 region affects the assimilation of seven carbohydrates, decreases the growth rate of the strain in minimal medium containing galacturonate or trehalose, and attenuates the virulence of E. coli BEN2908 for chickens.

* Corresponding author. Mailing address: Equipe de Pathologie Bactérienne, UR86, INRA, 37380 Nouzilly, France. Phone: 33 2 47 42 77 48. Fax: 33 2 47 42 77 74. E-mail: schouler{at}tours.inra.fr.

Journal of Bacteriology, February 2006, p. 977-987, Vol. 188, No. 3
0021-9193/06/$08.00+0     doi:10.1128/JB.188.3.977-987.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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