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Journal of Bacteriology, March 2006, p. 1817-1828, Vol. 188, No. 5
0021-9193/06/$08.00+0     doi:10.1128/JB.188.5.1817-1828.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Global Analysis of Heat Shock Response in Desulfovibrio vulgaris Hildenborough

S. R. Chhabra,1 Q. He,2 K. H. Huang,3 S. P. Gaucher,1 E. J. Alm,3 Z. He,2 M. Z. Hadi,1 T. C. Hazen,3 J. D. Wall,4 J. Zhou,2 A. P. Arkin,3 and A. K. Singh1*

Biosystems Research Department, Sandia National Laboratory, Livermore, California 94550,1 Environmental Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831,2 Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720,3 Biochemistry and Molecular Microbiology and Immunology Departments, University of Missouri—Columbia, Columbia, Missouri 652114

Received 23 September 2005/ Accepted 12 December 2005

Desulfovibrio vulgaris Hildenborough belongs to a class of sulfate-reducing bacteria (SRB) and is found ubiquitously in nature. Given the importance of SRB-mediated reduction for bioremediation of metal ion contaminants, ongoing research on D. vulgaris has been in the direction of elucidating regulatory mechanisms for this organism under a variety of stress conditions. This work presents a global view of this organism's response to elevated growth temperature using whole-cell transcriptomics and proteomics tools. Transcriptional response (1.7-fold change or greater; Z ≥ 1.5) ranged from 1,135 genes at 15 min to 1,463 genes at 120 min for a temperature up-shift of 13°C from a growth temperature of 37°C for this organism and suggested both direct and indirect modes of heat sensing. Clusters of orthologous group categories that were significantly affected included posttranslational modifications; protein turnover and chaperones (up-regulated); energy production and conversion (down-regulated), nucleotide transport, metabolism (down-regulated), and translation; ribosomal structure; and biogenesis (down-regulated). Analysis of the genome sequence revealed the presence of features of both negative and positive regulation which included the CIRCE element and promoter sequences corresponding to the alternate sigma factors {sigma}32 and {sigma}54. While mechanisms of heat shock control for some genes appeared to coincide with those established for Escherichia coli and Bacillus subtilis, the presence of unique control schemes for several other genes was also evident. Analysis of protein expression levels using differential in-gel electrophoresis suggested good agreement with transcriptional profiles of several heat shock proteins, including DnaK (DVU0811), HtpG (DVU2643), HtrA (DVU1468), and AhpC (DVU2247). The proteomics study also suggested the possibility of posttranslational modifications in the chaperones DnaK, AhpC, GroES (DVU1977), and GroEL (DVU1976) and also several periplasmic ABC transporters.

* Corresponding author. Mailing address: Biosystems Research Department, Mailstop 9292, Sandia National Laboratory, 7011 East Ave., Livermore, CA 94550. Phone: (925) 294-1260. Fax: (925) 294-3020. E-mail: aksingh{at}sandia.gov.

Journal of Bacteriology, March 2006, p. 1817-1828, Vol. 188, No. 5
0021-9193/06/$08.00+0     doi:10.1128/JB.188.5.1817-1828.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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