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Journal of Bacteriology, March 2006, p. 1999-2013, Vol. 188, No. 5
0021-9193/06/$08.00+0     doi:10.1128/JB.188.5.1999-2013.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

The clc Element of Pseudomonas sp. Strain B13, a Genomic Island with Various Catabolic Properties

Muriel Gaillard,1 Tatiana Vallaeys,2 Frank Jörg Vorhölter,3 Marco Minoia,1 Christoph Werlen,4 Vladimir Sentchilo,4 Alfred Pühler,3 and Jan Roelof van der Meer1*

Department of Fundamental Microbiology, University of Lausanne, CH-1015 Lausanne, Switzerland,1 INRA-INAPG, 78850 Thiverval Grignon, France,2 Department of Genetics, Faculty of Biology, Bielefeld University, D-33594 Bielefeld, Germany,3 Swiss Federal Institute of Aquatic Science and Technology (Eawag), CH-8600 Dübendorf, Switzerland4

Received 30 November 2005/ Accepted 19 December 2005

Pseudomonas sp. strain B13 is a bacterium known to degrade chloroaromatic compounds. The properties to use 3- and 4-chlorocatechol are determined by a self-transferable DNA element, the clc element, which normally resides at two locations in the cell's chromosome. Here we report the complete nucleotide sequence of the clc element, demonstrating the unique catabolic properties while showing its relatedness to genomic islands and integrative and conjugative elements rather than to other known catabolic plasmids. As far as catabolic functions, the clc element harbored, in addition to the genes for chlorocatechol degradation, a complete functional operon for 2-aminophenol degradation and genes for a putative aromatic compound transport protein and for a multicomponent aromatic ring dioxygenase similar to anthranilate hydroxylase. The genes for catabolic functions were inducible under various conditions, suggesting a network of catabolic pathway induction. For about half of the open reading frames (ORFs) on the clc element, no clear functional prediction could be given, although some indications were found for functions that were similar to plasmid conjugation. The region in which these ORFs were situated displayed a high overall conservation of nucleotide sequence and gene order to genomic regions in other recently completed bacterial genomes or to other genomic islands. Most notably, except for two discrete regions, the clc element was almost 100% identical over the whole length to a chromosomal region in Burkholderia xenovorans LB400. This indicates the dynamic evolution of this type of element and the continued transition between elements with a more pathogenic character and those with catabolic properties.

* Corresponding author. Mailing address: Department of Fundamental Microbiology, Batiment Biophore, University of Lausanne, CH-1015 Lausanne, Switzerland. Phone: 41 21 692 5630. Fax: 41 21 692 5605. E-mail: Janroelof.vandermeer{at}unil.ch.

Journal of Bacteriology, March 2006, p. 1999-2013, Vol. 188, No. 5
0021-9193/06/$08.00+0     doi:10.1128/JB.188.5.1999-2013.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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