This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Frye, J.
Right arrow Articles by Hughes, K. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Frye, J.
Right arrow Articles by Hughes, K. T.

 Previous Article  |  Next Article 

Journal of Bacteriology, March 2006, p. 2233-2243, Vol. 188, No. 6
0021-9193/06/$08.00+0     doi:10.1128/JB.188.6.2233-2243.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Identification of New Flagellar Genes of Salmonella enterica Serovar Typhimurium

Jonathan Frye,2 Joyce E. Karlinsey,1 Heather R. Felise,1 Bruz Marzolf,3 Naeem Dowidar,3 Michael McClelland,2 and Kelly T. Hughes1*

Department of Microbiology, Box 357242, University of Washington, Seattle, Washington 98195,1 Sidney Kimmel Cancer Center, 10835 Altman Row, San Diego, California 92121,2 The Institute for Systems Biology, 1441 N 34th Street, Seattle, Washington 981033

Received 21 September 2005/ Accepted 18 November 2005

RNA levels of flagellar genes in eight different genetic backgrounds were compared to that of the wild type by DNA microarray analysis. Cluster analysis identified new, potential flagellar genes, three putative methyl-accepting chemotaxis proteins, STM3138 (McpA), STM3152 (McpB), and STM3216(McpC), and a CheV homolog, STM2314, in Salmonella, that are not found in Escherichia coli. Isolation and characterization of Mud-lac insertions in cheV, mcpB, mcpC, and the previously uncharacterized aer locus of S. enterica serovar Typhimurium revealed them to be controlled by {sigma}28-dependent flagellar class 3 promoters. In addition, the srfABC operon previously isolated as an SsrB-regulated operon clustered with the flagellar class 2 operon and was determined to be under FlhDC control. The previously unclassified fliB gene, encoding flagellin methylase, clustered as a class 2 gene, which was verified using reporter fusions, and the fliB transcriptional start site was identified by primer extension analysis. RNA levels of all flagellar genes were elevated in flgM or fliT null strains. RNA levels of class 3 flagellar genes were elevated in a fliS null strain, while deletion of the fliY, fliZ, or flk gene did not affect flagellar RNA levels relative to those of the wild type. The cafA (RNase G) and yhjH genes clustered with flagellar class 3 transcribed genes. Null alleles in cheV, mcpA, mcpB, mcpC, and srfB did not affect motility, while deletion of yhjH did result in reduced motility compared to that of the wild type.

* Corresponding author. Mailing address: Department of Microbiology, Box 357242, University of Washington, Seattle, WA 98195. Phone: (801) 581-6517. Fax: (801) 581-4668. E-mail: Hughes{at}biology.utah.edu.

Journal of Bacteriology, March 2006, p. 2233-2243, Vol. 188, No. 6
0021-9193/06/$08.00+0     doi:10.1128/JB.188.6.2233-2243.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lowenthal, A. C., Simon, C., Fair, A. S., Mehmood, K., Terry, K., Anastasia, S., Ottemann, K. M. (2009). A fixed-time diffusion analysis method determines that the three cheV genes of Helicobacter pylori differentially affect motility. Microbiology 155: 1181-1191 [Abstract] [Full Text]  
  • Sommerfeldt, N., Possling, A., Becker, G., Pesavento, C., Tschowri, N., Hengge, R. (2009). Gene expression patterns and differential input into curli fimbriae regulation of all GGDEF/EAL domain proteins in Escherichia coli. Microbiology 155: 1318-1331 [Abstract] [Full Text]  
  • Pesavento, C., Becker, G., Sommerfeldt, N., Possling, A., Tschowri, N., Mehlis, A., Hengge, R. (2008). Inverse regulatory coordination of motility and curli-mediated adhesion in Escherichia coli. Genes Dev. 22: 2434-2446 [Abstract] [Full Text]  
  • Saini, S., Brown, J. D., Aldridge, P. D., Rao, C. V. (2008). FliZ Is a Posttranslational Activator of FlhD4C2-Dependent Flagellar Gene Expression. J. Bacteriol. 190: 4979-4988 [Abstract] [Full Text]  
  • Wolfe, A. J., Visick, K. L. (2008). Get the Message Out: Cyclic-Di-GMP Regulates Multiple Levels of Flagellum-Based Motility. J. Bacteriol. 190: 463-475 [Full Text]  
  • Bresolin, G., Trcek, J., Scherer, S., Fuchs, T. M. (2008). Presence of a functional flagellar cluster Flag-2 and low-temperature expression of flagellar genes in Yersinia enterocolitica W22703. Microbiology 154: 196-206 [Abstract] [Full Text]  
  • Lin, D., Rao, C. V., Slauch, J. M. (2008). The Salmonella SPI1 Type Three Secretion System Responds to Periplasmic Disulfide Bond Status via the Flagellar Apparatus and the RcsCDB System. J. Bacteriol. 190: 87-97 [Abstract] [Full Text]  
  • Wang, Q., Zhao, Y., McClelland, M., Harshey, R. M. (2007). The RcsCDB Signaling System and Swarming Motility in Salmonella enterica Serovar Typhimurium: Dual Regulation of Flagellar and SPI-2 Virulence Genes. J. Bacteriol. 189: 8447-8457 [Abstract] [Full Text]  
  • Claret, L., Miquel, S., Vieille, N., Ryjenkov, D. A., Gomelsky, M., Darfeuille-Michaud, A. (2007). The Flagellar Sigma Factor FliA Regulates Adhesion and Invasion of Crohn Disease-associated Escherichia coli via a Cyclic Dimeric GMP-dependent Pathway. J. Biol. Chem. 282: 33275-33283 [Abstract] [Full Text]  
  • Zhao, K., Liu, M., Burgess, R. R. (2007). Adaptation in bacterial flagellar and motility systems: from regulon members to 'foraging'-like behavior in E. coli. Nucleic Acids Res 35: 4441-4452 [Abstract] [Full Text]  
  • Thijs, I. M. V., De Keersmaecker, S. C. J., Fadda, A., Engelen, K., Zhao, H., McClelland, M., Marchal, K., Vanderleyden, J. (2007). Delineation of the Salmonella enterica Serovar Typhimurium HilA Regulon through Genome-Wide Location and Transcript Analysis. J. Bacteriol. 189: 4587-4596 [Abstract] [Full Text]  
  • Simm, R., Lusch, A., Kader, A., Andersson, M., Romling, U. (2007). Role of EAL-Containing Proteins in Multicellular Behavior of Salmonella enterica Serovar Typhimurium. J. Bacteriol. 189: 3613-3623 [Abstract] [Full Text]  
  • Fink, R. C., Evans, M. R., Porwollik, S., Vazquez-Torres, A., Jones-Carson, J., Troxell, B., Libby, S. J., McClelland, M., Hassan, H. M. (2007). FNR Is a Global Regulator of Virulence and Anaerobic Metabolism in Salmonella enterica Serovar Typhimurium (ATCC 14028s). J. Bacteriol. 189: 2262-2273 [Abstract] [Full Text]  
  • Coombes, B. K., Lowden, M. J., Bishop, J. L., Wickham, M. E., Brown, N. F., Duong, N., Osborne, S., Gal-Mor, O., Finlay, B. B. (2007). SseL Is a Salmonella-Specific Translocated Effector Integrated into the SsrB-Controlled Salmonella Pathogenicity Island 2 Type III Secretion System. Infect. Immun. 75: 574-580 [Abstract] [Full Text]  
  • Yu, H. H. Y., Kibler, D., Tan, M. (2006). In Silico Prediction and Functional Validation of {sigma}28-Regulated Genes in Chlamydia and Escherichia coli. J. Bacteriol. 188: 8206-8212 [Abstract] [Full Text]  
  • Wang, Q., Mariconda, S., Suzuki, A., McClelland, M., Harshey, R. M. (2006). Uncovering a Large Set of Genes That Affect Surface Motility in Salmonella enterica Serovar Typhimurium. J. Bacteriol. 188: 7981-7984 [Abstract] [Full Text]  
  • Shen, L., Feng, X., Yuan, Y., Luo, X., Hatch, T. P., Hughes, K. T., Liu, J. S., Zhang, Y.-x. (2006). Selective Promoter Recognition by Chlamydial {sigma}28 Holoenzyme. J. Bacteriol. 188: 7364-7377 [Abstract] [Full Text]  
  • Ryjenkov, D. A., Simm, R., Romling, U., Gomelsky, M. (2006). The PilZ Domain Is a Receptor for the Second Messenger c-di-GMP: THE PilZ DOMAIN PROTEIN YcgR CONTROLS MOTILITY IN ENTEROBACTERIA. J. Biol. Chem. 281: 30310-30314 [Abstract] [Full Text]  
  • Aldridge, P. D., Karlinsey, J. E., Aldridge, C., Birchall, C., Thompson, D., Yagasaki, J., Hughes, K. T. (2006). The flagellar-specific transcription factor, {sigma}28, is the Type III secretion chaperone for the flagellar-specific anti-{sigma}28 factor FlgM.. Genes Dev. 20: 2315-2326 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»