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Journal of Bacteriology, April 2006, p. 3110-3115, Vol. 188, No. 8
0021-9193/06/$08.00+0     doi:10.1128/JB.188.8.3110-3115.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

EspF of Enteropathogenic Escherichia coli Binds Sorting Nexin 9

Oliver Marchès,1 Miranda Batchelor,1 Robert K. Shaw,3 Amit Patel,4 Nicola Cummings,4 Takeshi Nagai,5 Chihiro Sasakawa,5 Sven R. Carlsson,6 Richard Lundmark,6 Celine Cougoule,1,2 Emmanuelle Caron,1,2 Stuart Knutton,3 Ian Connerton,4 and Gad Frankel1*

Division of Cell and Molecular Biology,1 Centre for Molecular Microbiology and Infection, Imperial College London, London SW7 2AZ, United Kingdom,2 Institute of Child Health, University of Birmingham, Birmingham B4 6NH, United Kingdom,3 Division of Food Sciences, School of Biosciences, University of Nottingham, Loughborough LE 12 5DR, United Kingdom,4 Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan,5 Department of Medical Biochemistry and Biophysics, Umea University, S-901 87, Umea, Sweden6

Received 25 November 2005/ Accepted 6 February 2006

EspF of enteropathogenic Escherichia coli targets mitochondria and subverts a number of cellular functions. EspF consists of six putative Src homology 3 (SH3) domain binding motifs. In this study we identified sorting nexin 9 (SNX9) as a host cell EspF binding partner protein, which binds EspF via its amino-terminal SH3 region. Coimmunoprecipitation and confocal microscopy showed specific EspF-SNX9 interaction and non-mitochondrial protein colocalization in infected epithelial cells.

* Corresponding author. Mailing address: Flowers Building, Imperial College London, London SW7 2AZ, United Kingdom. Phone: 44 (0)20 75945253. Fax: 44 (0)20 75945253. E-mail: g.frankel{at}imperial.ac.uk.

Journal of Bacteriology, April 2006, p. 3110-3115, Vol. 188, No. 8
0021-9193/06/$08.00+0     doi:10.1128/JB.188.8.3110-3115.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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