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Journal of Bacteriology, May 2006, p. 3264-3272, Vol. 188, No. 9
0021-9193/06/$08.00+0     doi:10.1128/JB.188.9.3264-3272.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Specific Modification of a Na+ Binding Site in NADH:Quinone Oxidoreductase from Klebsiella pneumoniae with Dicyclohexylcarbodiimide{dagger} ,{ddagger}

Irini Vgenopoulou, Anja C. Gemperli, and Julia Steuber*

Biochemisches Institut, Universität Zürich, Winterthurerstr. 190, CH-8057 Zürich, Switzerland

Received 16 September 2005/ Accepted 13 February 2006

The respiratory NADH:quinone oxidoreductase (complex I) (NDH-1) is a multisubunit enzyme that translocates protons (or in some cases Na+) across energy-conserving membranes from bacteria or mitochondria. We studied the reaction of the Na+-translocating complex I from the enterobacterium Klebsiella pneumoniae with N,N'-dicyclohexylcarbodiimide (DCCD), with the aim of identifying a subunit critical for Na+ binding. At low Na+ concentrations (0.6 mM), DCCD inhibited both quinone reduction and Na+ transport by NDH-1 concurrent with the covalent modification of a 30-kDa polypeptide. In the presence of 50 mM Na+, NDH-1 was protected from inhibition by DCCD, and the modification of the 30-kDa polypeptide with [14C]DCCD was prevented, indicating that Na+ and DCCD competed for the binding to a critical carboxyl group in NDH-1. The 30-kDa polypeptide was assigned to NuoH, the homologue of the ND1 subunit from mitochondrial complex I. It is proposed that Na+ binds to the NuoH subunit during NADH-driven Na+ transport by NDH-1.

* Corresponding author. Mailing address: Biochemisches Institut, Winterthurerstr. 190, Universität Zürich, CH-8057 Zürich, Switzerland. Phone: 41 1 635 5567. Fax: 41 1 635 5712. E-mail: steuber{at}bioc.unizh.ch.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

{ddagger} Dedicated to Peter Dimroth on the occasion of his 65th birthday.

Journal of Bacteriology, May 2006, p. 3264-3272, Vol. 188, No. 9
0021-9193/06/$08.00+0     doi:10.1128/JB.188.9.3264-3272.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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