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Journal of Bacteriology, May 2006, p. 3409-3411, Vol. 188, No. 9
0021-9193/06/$08.00+0     doi:10.1128/JB.188.9.3409-3411.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Role of Secondary Attachment Sites in Changing the Specificity of Site-Specific Recombination

Edit Rutkai,1 Andrea György,1 László Dorgai,1* and Robert A. Weisberg2

Department of Molecular Biotechnology, Bay Zoltán Institute for Biotechnology, Szeged, Hungary,1 Section on Microbial Genetics, Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland2

Received 24 October 2005/ Accepted 10 February 2006

We previously proposed that lambdoid phages change their insertion specificity by adapting their integrases to sequences found in secondary attachment sites. To test this model, we quantified recombination between partners that carried sequences from secondary attachment sites catalyzed by wild-type and by mutant integrases with altered specificities. The results are consistent with the model, and indicate differential core site usage in excision and integration.

* Corresponding author. Present address: Biocenter Ltd., Temesvári krt. 62, Szeged 6726, Hungary. Phone: 36 30 567 8941. Fax: 36 62 599 751. E-mail: dorgai{at}biocenter.hu.

Journal of Bacteriology, May 2006, p. 3409-3411, Vol. 188, No. 9
0021-9193/06/$08.00+0     doi:10.1128/JB.188.9.3409-3411.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Serra-Moreno, R., Jofre, J., Muniesa, M. (2007). Insertion Site Occupancy by stx2 Bacteriophages Depends on the Locus Availability of the Host Strain Chromosome. J. Bacteriol. 189: 6645-6654 [Abstract] [Full Text]  


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