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Journal of Bacteriology, May 2007, p. 3751-3758, Vol. 189, No. 10
0021-9193/07/$08.00+0     doi:10.1128/JB.01722-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Expression Analysis of Multiple dnaK Genes in the Cyanobacterium Synechococcus elongatus PCC 7942{triangledown} ,{dagger}

Masumi Sato, Kaori Nimura-Matsune, Satoru Watanabe, Taku Chibazakura, and Hirofumi Yoshikawa*

Department of Bioscience, Tokyo University of Agriculture, Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan

Received 8 November 2006/ Accepted 27 February 2007

We analyzed the stress responses of three dnaK homologues (dnaK1, dnaK2, and dnaK3) in the cyanobacterium Synechococcus elongatus PCC 7942. A reporter assay showed that under stress conditions the expression of only the dnaK2 gene was induced, suggesting a functional assignment of these homologues. RNA blot hybridization indicated a typical stress response of dnaK2 to heat and high-light stress. Primer extension mapping showed that dnaK2 was transcribed from similar sites under various stress conditions. Although no known sequence motif was detected in the upstream region, a 20-bp sequence element was highly conserved in dnaK2; it was essential not only for the stress induction but also for the basal expression of dnaK2. The ubiquitous upstream localization of this element in each heat shock gene suggests its important role in the cyanobacterial stress response.

* Corresponding author. Mailing address: Department of Bioscience, Tokyo University of Agriculture, Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan. Phone: 81 (3) 5477 2758. Fax: 81 (3) 5477 2668. E-mail: hiyoshik{at}nodai.ac.jp

{triangledown} Published ahead of print on 9 March 2007.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, May 2007, p. 3751-3758, Vol. 189, No. 10
0021-9193/07/$08.00+0     doi:10.1128/JB.01722-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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