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Journal of Bacteriology, May 2007, p. 3918-3921, Vol. 189, No. 10
0021-9193/07/$08.00+0     doi:10.1128/JB.01863-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Target Site Selection of Pseudomonas putida Transposon Tn4652{triangledown} ,{dagger}

Paula Ann Kivistik, Maia Kivisaar, and Rita Hõrak*

Estonian Biocentre and Institute of Molecular and Cell Biology, Tartu University, 51010 Tartu, Estonia

Received 12 December 2006/ Accepted 2 March 2007

We analyzed the target preferences of a Tn3 family transposon Tn4652. Alignment of 93 different insertion sites revealed a consensus sequence which resembles that of Tn3, indicating that despite a low similarity between Tn4652 and Tn3 transposases, their target site recognition is conserved.

* Corresponding author. Mailing address: Estonian Biocentre and Institute of Molecular and Cell Biology, Tartu University, 23 Riia Street, 51010, Tartu, Estonia. Phone: 372 7 375015. Fax: 372 7 420286. E-mail: rhorak{at}ebc.ee

{triangledown} Published ahead of print on 9 March 2007.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, May 2007, p. 3918-3921, Vol. 189, No. 10
0021-9193/07/$08.00+0     doi:10.1128/JB.01863-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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