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Journal of Bacteriology, June 2007, p. 4353-4358, Vol. 189, No. 12
0021-9193/07/$08.00+0     doi:10.1128/JB.00193-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Role of RNA Structure and Susceptibility to RNase E in Regulation of a Cold Shock mRNA, cspA mRNA{triangledown}

Janet S. Hankins, Christopher Zappavigna, Annie Prud'homme-Généreux, and George A. Mackie*

Department of Biochemistry and Molecular Biology, University of British Columbia, Life Sciences Centre, 2350 Health Sciences Mall, Vancouver, British Columbia, Canada V6T 1Z3

Received 5 February 2007/ Accepted 30 March 2007

Degradation of the cspA mRNA in vivo is very rapid at temperatures greater than 30°C and is moderately dependent on RNase E. Investigations in vitro show that degradosomes prepared from normal or cold-shocked cultures cleave the cspA mRNA preferentially at a single site in vitro between two stem-loops ~24 residues 3' to the termination codon and ~31 residues from the 3' end. The site of cleavage is independent of the temperature and largely independent of the phosphorylation status of the 5' end of cspA mRNA. A 5' stem-loop, potential occlusion of the initiation and termination codons, temperature-dependent translational efficiency, and the position of the RNase E cleavage site can explain the differential stability of the cspA mRNA.

* Corresponding author. Mailing address: Department of Biochemistry and Molecular Biology, University of British Columbia, Life Sciences Centre, 2350 Health Sciences Mall, Vancouver, British Columbia, Canada V6T 1Z3. Phone: (604) 822-5943. Fax: (604)-822-5227. E-mail: gamackie{at}interchange.ubc.ca

{triangledown} Published ahead of print on 6 April 2007.

Journal of Bacteriology, June 2007, p. 4353-4358, Vol. 189, No. 12
0021-9193/07/$08.00+0     doi:10.1128/JB.00193-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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