This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Duan, K.
Right arrow Articles by Surette, M. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Duan, K.
Right arrow Articles by Surette, M. G.

 Previous Article  |  Next Article 

Journal of Bacteriology, July 2007, p. 4827-4836, Vol. 189, No. 13
0021-9193/07/$08.00+0     doi:10.1128/JB.00043-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Environmental Regulation of Pseudomonas aeruginosa PAO1 Las and Rhl Quorum-Sensing Systems{triangledown}

Kangmin Duan1,3 and Michael G. Surette1,2*

Department of Microbiology and Infectious Diseases,1 Biochemistry and Molecular Biology, University of Calgary, Calgary, Alberta, Canada T2N 4N1,2 Molecular Microbiology Laboratory, Faculty of Life Sciences, Northwest University, Xi'an 710069, China3

Received 9 January 2007/ Accepted 11 April 2007

The lasI-lasR and the rhlI-rhlR quorum-sensing systems in Pseudomonas aeruginosa regulate the expression of numerous cellular and secreted virulence factor genes and play important roles in the development of biofilms. The las and rhl systems themselves are known to be directly or indirectly regulated by a number of transcriptional regulators, and consequently, their expression is sensitive to environmental conditions. In this report, the activities of these two quorum-sensing systems have been examined systematically under 46 growth conditions, and the regulation by environmental conditions has been investigated. The relative timing and strength of expression of these two systems varied significantly under different conditions, which contrasts with the notion of a preset hierarchy with these two systems in P. aeruginosa. Depending on the growth conditions, the correlation between each synthase and its cognate transcriptional regulator also varied, suggesting that the transcription of these genes independently allows for further fine tuning of each system. Finally, we observe that the activities of both the lasI-lasR and the rhlI-rhlR quorum-sensing systems were dramatically enhanced in the presence of extracts of sputum samples from cystic fibrosis patients.

* Corresponding author. Mailing address: Faculty of Medicine, Department of Microbiology and Infectious Diseases, Biochemistry and Molecular Biology, University of Calgary, 3330 Hospital Dr. NW, Calgary T2N 4N1, Canada. Phone: (403) 220-2744. Fax: (403) 270-2772. E-mail: surette{at}ucalgary.ca

{triangledown} Published ahead of print on 20 April 2007.

Journal of Bacteriology, July 2007, p. 4827-4836, Vol. 189, No. 13
0021-9193/07/$08.00+0     doi:10.1128/JB.00043-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Steindler, L., Bertani, I., De Sordi, L., Schwager, S., Eberl, L., Venturi, V. (2009). LasI/R and RhlI/R Quorum Sensing in a Strain of Pseudomonas aeruginosa Beneficial to Plants. Appl. Environ. Microbiol. 75: 5131-5140 [Abstract] [Full Text]  
  • Zhang, M., Jiao, X.-d., Hu, Y.-h., Sun, L. (2009). Attenuation of Edwardsiella tarda Virulence by Small Peptides That Interfere with LuxS/Autoinducer Type 2 Quorum Sensing. Appl. Environ. Microbiol. 75: 3882-3890 [Abstract] [Full Text]  
  • Liang, H., Li, L., Dong, Z., Surette, M. G., Duan, K. (2008). The YebC Family Protein PA0964 Negatively Regulates the Pseudomonas aeruginosa Quinolone Signal System and Pyocyanin Production. J. Bacteriol. 190: 6217-6227 [Abstract] [Full Text]  
  • Patriquin, G. M., Banin, E., Gilmour, C., Tuchman, R., Greenberg, E. P., Poole, K. (2008). Influence of Quorum Sensing and Iron on Twitching Motility and Biofilm Formation in Pseudomonas aeruginosa. J. Bacteriol. 190: 662-671 [Abstract] [Full Text]  
  • Kirisits, M. J., Margolis, J. J., Purevdorj-Gage, B. L., Vaughan, B., Chopp, D. L., Stoodley, P., Parsek, M. R. (2007). Influence of the Hydrodynamic Environment on Quorum Sensing in Pseudomonas aeruginosa Biofilms. J. Bacteriol. 189: 8357-8360 [Abstract] [Full Text]  
  • Morici, L. A., Carterson, A. J., Wagner, V. E., Frisk, A., Schurr, J. R., zu Bentrup, K. H., Hassett, D. J., Iglewski, B. H., Sauer, K., Schurr, M. J. (2007). Pseudomonas aeruginosa AlgR Represses the Rhl Quorum-Sensing System in a Biofilm-Specific Manner. J. Bacteriol. 189: 7752-7764 [Abstract] [Full Text]  
  • Sandoz, K. M., Mitzimberg, S. M., Schuster, M. (2007). From the Cover: Social cheating in Pseudomonas aeruginosa quorum sensing. Proc. Natl. Acad. Sci. USA 104: 15876-15881 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»