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Journal of Bacteriology, July 2007, p. 5130-5141, Vol. 189, No. 14
0021-9193/07/$08.00+0     doi:10.1128/JB.00145-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

The Haemophilus influenzae hFbpABC Fe3+ Transporter: Analysis of the Membrane Permease and Development of a Gallium-Based Screen for Mutants{triangledown}

Damon S. Anderson,1,{dagger} Pratima Adhikari,1 Katherine D. Weaver,2 Alvin L. Crumbliss,2 and Timothy A. Mietzner1*

Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261,1 Department of Chemistry, Duke University, Durham, North Carolina 27708-03462

Received 29 January 2007/ Accepted 27 April 2007

The obligate human pathogen Haemophilus influenzae utilizes a siderophore-independent (free) Fe3+ transport system to obtain this essential element from the host iron-binding protein transferrin. The hFbpABC transporter is a binding protein-dependent ABC transporter that functions to shuttle (free) Fe3+ through the periplasm and across the inner membrane of H. influenzae. This investigation focuses on the structure and function of the hFbpB membrane permease component of the transporter, a protein that has eluded prior characterization. Based on multiple-sequence alignments between permease orthologs, a series of site-directed mutations targeted at residues within the two conserved permease motifs were generated. The hFbpABC transporter was expressed in a siderophore-deficient Escherichia coli background, and effects of mutations were analyzed using growth rescue and radiolabeled 55Fe3+ transport assays. Results demonstrate that mutation of the invariant glycine (G418A) within motif 2 led to attenuated transport activity, while mutation of the invariant glycine (G155A/V/E) within motif 1 had no discernible effect on activity. Individual mutations of well-conserved leucines (L154D and L417D) led to attenuated and null transport activities, respectively. As a complement to site-directed methods, a mutant screen based on resistance to the toxic iron analog gallium, an hFbpABC inhibitor, was devised. The screen led to the identification of several significant hFbpB mutations; V497I, I174F, and S475I led to null transport activities, while S146Y resulted in attenuated activity. Significant residues were mapped to a topological model of the hFbpB permease, and the implications of mutations are discussed in light of structural and functional data from related ABC transporters.

* Corresponding author. Mailing address: Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, E1240 Biomedical Science Tower, Lothrop Street, Pittsburgh, PA 15261. Phone: (412) 648-9244. Fax: (412) 624-1401. E-mail: mietzner{at}mgb.pitt.edu

{triangledown} Published ahead of print on 11 May 2007.

{dagger} Present address: Molecular Cardiology Research Institute, Tufts-New England Medical Center, Boston, MA 02111.

Journal of Bacteriology, July 2007, p. 5130-5141, Vol. 189, No. 14
0021-9193/07/$08.00+0     doi:10.1128/JB.00145-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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