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Journal of Bacteriology, August 2007, p. 5762-5772, Vol. 189, No. 15
0021-9193/07/$08.00+0     doi:10.1128/JB.00371-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Deletion of the parA (soj) Homologue in Pseudomonas aeruginosa Causes ParB Instability and Affects Growth Rate, Chromosome Segregation, and Motility{triangledown}

Krzysztof Lasocki,1,{dagger} Aneta A. Bartosik,1 Jolanta Mierzejewska,1 Christopher M. Thomas,2 and Grazyna Jagura-Burdzy1*

The Institute of Biochemistry and Biophysics, PAS, 02-106 Warsaw, Pawinskiego 5A, Poland,1 School of Biosciences, The University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdon2

Received 12 March 2007/ Accepted 22 May 2007

The parA and parB genes of Pseudomonas aeruginosa are located approximately 8 kb anticlockwise from oriC. ParA is a cytosolic protein present at a level of around 600 molecules per cell in exponential phase, but the level drops about fivefold in stationary phase. Overproduction of full-length ParA or the N-terminal 85 amino acids severely inhibits growth of P. aeruginosa and P. putida. Both inactivation of parA and overexpression of parA in trans in P. aeruginosa also lead to accumulation of anucleate cells and changes in motility. Inactivation of parA also increases the turnover rate (degradation) of ParB. This may provide a mechanism for controlling the level of ParB in response to the growth rate and expression of the parAB operon.

* Corresponding author. Mailing address: The Institute of Biochemistry and Biophysics, PAS, 02-106 Warsaw, Pawinskiego 5A, Poland. Phone: 48 22 823 71 92. Fax: 48 22 658 46 36. E-mail: gjburdzy{at}ibb.waw.pl

{triangledown} Published ahead of print on 1 June 2007.

{dagger} Present address: Department of Pathology, Tufts University School of Medicine, Boston, MA 02111.

Journal of Bacteriology, August 2007, p. 5762-5772, Vol. 189, No. 15
0021-9193/07/$08.00+0     doi:10.1128/JB.00371-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Bartosik, A. A., Mierzejewska, J., Thomas, C. M., Jagura-Burdzy, G. (2009). ParB deficiency in Pseudomonas aeruginosa destabilizes the partner protein ParA and affects a variety of physiological parameters. Microbiology 155: 1080-1092 [Abstract] [Full Text]  
  • Toro, E., Hong, S.-H., McAdams, H. H., Shapiro, L. (2008). Caulobacter requires a dedicated mechanism to initiate chromosome segregation. Proc. Natl. Acad. Sci. USA 105: 15435-15440 [Abstract] [Full Text]  
  • Livny, J., Yamaichi, Y., Waldor, M. K. (2007). Distribution of Centromere-Like parS Sites in Bacteria: Insights from Comparative Genomics. J. Bacteriol. 189: 8693-8703 [Abstract] [Full Text]  


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