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Journal of Bacteriology, August 2007, p. 5792-5802, Vol. 189, No. 16
0021-9193/07/$08.00+0     doi:10.1128/JB.00540-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Genomic Characterization of the Filamentous Integrative Bacteriophages {phi}RSS1 and {phi}RSM1, Which Infect Ralstonia solanacearum{triangledown}

Takeru Kawasaki, Shoko Nagata, Akiko Fujiwara, Hideki Satsuma, Makoto Fujie, Shoji Usami, and Takashi Yamada*

Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima 739-8530, Japan

Received 10 April 2007/ Accepted 30 May 2007

The genomic DNA sequences were determined for two filamentous integrative bacteriophages, {phi}RSS1 and {phi}RSM1, of the phytopathogen Ralstonia solanacearum. The 6,662-base sequence of {phi}RSS1 contained 11 open reading frames (ORFs). In the databases, this sequence showed high homology (95% identity) to the circular double-stranded DNA plasmid pJTPS1 (6,633 bp) isolated from a spontaneously occurring avirulent mutant of R. solanacearum. Two major differences between the two sequences were observed within {phi}RSS1 ORF7, corresponding to pIII, a minor coat protein required for host adsorption, and at the {phi}RSS1 intergenic (IG) region. The 9,004-base sequence of {phi}RSM1 showed 12 ORFs located on the same strand (plus strand) and 2 ORFs on the opposite strand. Compared with Ff-type phages, two insertions are obvious in the {phi}RSM1 replication module. Genomic DNA fragments containing the {phi}RSM integration junctions were cloned and sequenced from {phi}RSM lysogenic strain R. solanacearum MAFF211270. The att core sequence was identified as 5'-TGGCGGAGAGGGT-3', corresponding to the 3' end of the serine tRNA (UCG) gene. Interestingly, ORF14, located next to the attP site on the {phi}RSM1 genome, showed high amino acid sequence homology with bacterial DNA recombinases and resolvases, different from XerCD recombinases. attP of {phi}RSS1 is within a sequence element of the IG region.

* Corresponding author. Mailing address: Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima 739-8530, Japan. Phone and fax: 81-82-424-7752. E-mail: tayamad{at}hiroshima-u.ac.jp

{triangledown} Published ahead of print on 8 June 2007.

Journal of Bacteriology, August 2007, p. 5792-5802, Vol. 189, No. 16
0021-9193/07/$08.00+0     doi:10.1128/JB.00540-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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