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Journal of Bacteriology, August 2007, p. 5808-5815, Vol. 189, No. 16
0021-9193/07/$08.00+0     doi:10.1128/JB.00655-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Complementation Analysis of the Cold-Sensitive Phenotype of the Escherichia coli csdA Deletion Strain{triangledown}

Naoki Awano,{dagger} Chunying Xu,{dagger} Haiping Ke, Koichi Inoue, Masayori Inouye, and Sangita Phadtare*

Department of Biochemistry, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, New Jersey 08854

Received 26 April 2007/ Accepted 30 May 2007

The cold shock response of Escherichia coli is elicited by downshift of temperature from 37°C to 15°C and is characterized by induction of several cold shock proteins, including CsdA, during the acclimation phase. CsdA, a DEAD-box protein, has been proposed to participate in a variety of processes, such as ribosome biogenesis, mRNA decay, translation initiation, and gene regulation. It is not clear which of the functions of CsdA play a role in its essential cold shock function or whether all do, and so far no protein has been shown to complement its function in vivo. Our screening of an E. coli genomic library for an in vivo counterpart of CsdA that can compensate for its absence at low temperature revealed only one protein, RhlE, another DEAD-box RNA helicase. We also observed that although not detected in our genetic screening, two cold shock-inducible proteins, namely, CspA, an RNA chaperone, and RNase R, an exonuclease, can also complement the cold shock function of CsdA. Interestingly, the absence of CsdA and RNase R leads to increased sensitivity of the cells to even moderate temperature downshifts. The correlation between the helicase activity of CsdA and the stability of mRNAs of cold-inducible genes was shown using cspA mRNA, which was significantly stabilized in the {Delta}csdA cells, an effect counteracted by overexpression of wild-type CsdA or RNase R but not by that of the helicase-deficient mutant of CsdA. These results suggest that the primary role of CsdA in cold acclimation of cells is in mRNA decay and that its helicase activity is pivotal for promoting degradation of mRNAs stabilized at low temperature.

* Corresponding author. Mailing address: Department of Biochemistry, Robert Wood Johnson Medical School, 675 Hoes Lane, Piscataway, NJ 08854. Phone: (732) 235-4116. Fax: (732) 235-4559. E-mail: phadtasa{at}umdnj.edu

{triangledown} Published ahead of print on 8 June 2007.

{dagger} N.A. and C.X. contributed equally to the work.

Journal of Bacteriology, August 2007, p. 5808-5815, Vol. 189, No. 16
0021-9193/07/$08.00+0     doi:10.1128/JB.00655-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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