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Journal of Bacteriology, August 2007, p. 5903-5915, Vol. 189, No. 16
0021-9193/07/$08.00+0     doi:10.1128/JB.00257-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

A Genomic View of Sugar Transport in Mycobacterium smegmatis and Mycobacterium tuberculosis{triangledown}

Fritz Titgemeyer,1* Johannes Amon,1 Stephan Parche,1 Maysa Mahfoud,1 Johannes Bail,1 Maximilian Schlicht,1 Nadine Rehm,1 Dietmar Hillmann,1,2 Joachim Stephan,1 Britta Walter,1 Andreas Burkovski,1 and Michael Niederweis1,2*

Lehrstuhl für Mikrobiologie, Friedrich Alexander Universität Erlangen-Nürnberg, Staudtstr. 5, D-91058 Erlangen, Germany,1 Department of Microbiology, University of Alabama at Birmingham, 613 Bevill Biomedical Research Building, 845 19th Street South, Birmingham, Alabama 352942

Received 14 February 2007/ Accepted 22 May 2007

We present a comprehensive analysis of carbohydrate uptake systems of the soil bacterium Mycobacterium smegmatis and the human pathogen Mycobacterium tuberculosis. Our results show that M. smegmatis has 28 putative carbohydrate transporters. The majority of sugar transport systems (19/28) in M. smegmatis belong to the ATP-binding cassette (ABC) transporter family. In contrast to previous reports, we identified genes encoding all components of the phosphotransferase system (PTS), including permeases for fructose, glucose, and dihydroxyacetone, in M. smegmatis. It is anticipated that the PTS of M. smegmatis plays an important role in the global control of carbon metabolism similar to those of other bacteria. M. smegmatis further possesses one putative glycerol facilitator of the major intrinsic protein family, four sugar permeases of the major facilitator superfamily, one of which was assigned as a glucose transporter, and one galactose permease of the sodium solute superfamily. Our predictions were validated by gene expression, growth, and sugar transport analyses. Strikingly, we detected only five sugar permeases in the slow-growing species M. tuberculosis, two of which occur in M. smegmatis. Genes for a PTS are missing in M. tuberculosis. Our analysis thus brings the diversity of carbohydrate uptake systems of fast- and a slow-growing mycobacteria to light, which reflects the lifestyles of M. smegmatis and M. tuberculosis in their natural habitats, the soil and the human body, respectively.

* Corresponding author. Mailing address for Fritz Titgemeyer: Lehrstuhl für Mikrobiologie, Friedrich Alexander Universität Erlangen- Nürnberg, Staudtstr. 5, D-91058 Erlangen, Germany. Phone: 49 177 4824821. Fax: 49 9131 8528082. E-mail: fritz.titgemeyer{at}googlemail.com. Mailing address for Michael Niederweis: Department of Microbiology, University of Alabama at Birmingham, 613 Bevill Biomedical Research Building, 845 19th Street South, Birmingham, AL 35294. Phone: (205) 996 2711. Fax: (205) 934 9256. E-mail: mnieder{at}uab.edu

{triangledown} Published ahead of print on 8 June 2007.

Journal of Bacteriology, August 2007, p. 5903-5915, Vol. 189, No. 16
0021-9193/07/$08.00+0     doi:10.1128/JB.00257-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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