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Journal of Bacteriology, October 2007, p. 6816-6823, Vol. 189, No. 19
0021-9193/07/$08.00+0     doi:10.1128/JB.00910-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

The Amino Terminus of Bacillus subtilis TagB Possesses Separable Localization and Functional Properties{triangledown} ,{dagger}

Amit P. Bhavsar,{ddagger} Michael A. D'Elia, Tiffany D. Sahakian, and Eric D. Brown*

Antimicrobial Research Centre and Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario L8N 3Z5, Canada

Received 10 June 2007/ Accepted 20 July 2007

The function(s) of gram-positive wall teichoic acid is emerging with recent findings that it is an important virulence factor in the pathogen Staphylococcus aureus and that it is crucial to proper rod-shaped cell morphology of Bacillus subtilis. Despite its importance, our understanding of teichoic acid biosynthesis remains incomplete. The TagB protein has been implicated in the priming step of poly(glycerol phosphate) wall teichoic acid synthesis in B. subtilis. Work to date indicates that the TagB protein is localized to the membrane, where it adds a single glycerol phosphate residue to the nonreducing end of the undecaprenol-phosphate-linked N-acetylmannosamine-ß(1,4)-N-acetylglucosamine-1-phosphate. Thus, membrane association is critical to TagB function. In this work we elucidate the mechanism of TagB membrane localization. We report the identification of a membrane targeting determinant at the amino terminus of TagB that is necessary and sufficient for membrane localization. The putative amphipathicity of this membrane targeting determinant was characterized and shown to be required for TagB function but not localization. This work shows for the first time that the amino terminus of TagB mediates membrane targeting and protein function.

* Corresponding author. Mailing address: Department of Biochemistry and Biomedical Sciences, McMaster University, 1200 Main Street West, Hamilton, Ontario L8N 3Z5, Canada. Phone: (905) 525-9140, ext. 22392. Fax: (905) 522-9033. E-mail: ebrown{at}mcmaster.ca

{triangledown} Published ahead of print on 27 July 2007.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

{ddagger} Present address: Michael Smith Laboratories, University of British Columbia, 301-2185 East Mall Road, Vancouver, BC V6T 1Z4, Canada.

Journal of Bacteriology, October 2007, p. 6816-6823, Vol. 189, No. 19
0021-9193/07/$08.00+0     doi:10.1128/JB.00910-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Sewell, E. W. C., Pereira, M. P., Brown, E. D. (2009). The Wall Teichoic Acid Polymerase TagF Is Non-processive in Vitro and Amenable to Study Using Steady State Kinetic Analysis. J. Biol. Chem. 284: 21132-21138 [Abstract] [Full Text]  


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