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Journal of Bacteriology, October 2007, p. 7190-7194, Vol. 189, No. 20
0021-9193/07/$08.00+0     doi:10.1128/JB.00842-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Synergistic Interaction of Clostridium cellulovorans Cellulosomal Cellulases and HbpA{triangledown}

Satoshi Matsuoka,1 Hideaki Yukawa,2 Masayuki Inui,2 and Roy H. Doi1*

Section of Molecular and Cellular Biology, University of California, Davis, California,1 RITE Institute, Kyoto, Japan2

Received 30 May 2007/ Accepted 4 August 2007

Clostridium cellulovorans, an anaerobic bacterium, produces a small nonenzymatic protein called HbpA, which has a surface layer homology domain and a type I cohesin domain similar to those found in the cellulosomal scaffolding protein CbpA. In this study, we demonstrated that HbpA could bind to cell wall fragments from C. cellulovorans and insoluble polysaccharides and form a complex with cellulosomal cellulases endoglucanase B (EngB) and endoglucanase L (EngL). Synergistic degradative action of the cellulosomal cellulase and HbpA complexes was demonstrated on acid-swollen cellulose, Avicel, and corn fiber. We propose that HbpA functions to bind dockerin-containing cellulosomal enzymes to the cell surface and complements the activity of cellulosomes.

* Corresponding author. Mailing address: Section of Molecular and Cellular Biology, University of California, Davis, CA. Phone: (530) 752-3191. Fax: (530) 752-3085. E-mail: rhdoi{at}ucdavis.edu

{triangledown} Published ahead of print on 10 August 2007.

Journal of Bacteriology, October 2007, p. 7190-7194, Vol. 189, No. 20
0021-9193/07/$08.00+0     doi:10.1128/JB.00842-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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