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Journal of Bacteriology, November 2007, p. 7765-7773, Vol. 189, No. 21
0021-9193/07/$08.00+0     doi:10.1128/JB.00853-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

RegA Control of Bacteriochlorophyll and Carotenoid Synthesis in Rhodobacter capsulatus{triangledown} ,{dagger}

Jonathan Willett,1 James L. Smart,2 and Carl E. Bauer1*

Department of Biology, Indiana University, Bloomington, Indiana 47405,1 Department of Biology, University of Tennessee at Martin, Martin, Tennessee 382372

Received 1 June 2007/ Accepted 29 June 2007

We provide in vivo genetic and in vitro biochemical evidence that RegA directly regulates bacteriochlorophyll and carotenoid biosynthesis in Rhodobacter capsulatus. ß-Galactosidase expression assays with a RegA-disrupted strain containing reporter plasmids for Mg-protoporphyrin IX monomethyl ester oxidative cyclase (bchE), Mg-protoporphyrin IX chelatase (bchD), and phytoene dehydrogenase (crtI) demonstrate RegA is responsible for fourfold anaerobic induction of bchE, threefold induction of bchD, and twofold induction of crtI. Promoter mapping studies, coupled with DNase I protection assays, map the region of RegA binding to three sites in the bchE promoter region. Similar studies at the crtA and crtI promoters indicate that RegA binds to a single region equidistant from these divergent promoters. These results demonstrate that RegA is directly responsible for anaerobic induction of bacteriochlorophyll biosynthesis genes bchE, bchD, bchJ, bchI, bchG, and bchP and carotenoid biosynthesis genes crtI, crtB, and crtA.

* Corresponding author. Mailing address: Department of Biology, Jordan Hall, Bloomington, IN 47405. Phone: (812) 855-6595. Fax: (812) 856-4178. E-mail: cbauer{at}bio.indiana.edu

{triangledown} Published ahead of print on 6 July 2007.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, November 2007, p. 7765-7773, Vol. 189, No. 21
0021-9193/07/$08.00+0     doi:10.1128/JB.00853-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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