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Journal of Bacteriology, November 2007, p. 8215-8223, Vol. 189, No. 22
0021-9193/07/$08.00+0     doi:10.1128/JB.01039-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Identification of a Vibrio furnissii Oligopeptide Permease and Characterization of Its In Vitro Hemolytic Activity{triangledown} ,{dagger}

Tung-Kung Wu,* Yu-Kuo Wang, Yi-Chin Chen, Jen-Min Feng, Yen-Hsi Liu, and Ting-Yi Wang

Department of Biological Science and Technology, National Chiao Tung University, Hsin-Chu, Taiwan, 300, Republic of China

Received 1 July 2007/ Accepted 4 September 2007

We describe purification and characterization of an oligopeptide permease protein (Hly-OppA) from Vibrio furnissii that has multifaceted functions in solute binding, in in vitro hemolysis, in antibiotic resistance, and as a virulence factor in bacterial pathogenesis. The solute-binding function was revealed by N-terminal and internal peptide sequences of the purified protein and was confirmed by discernible effects on oligopeptide binding, by accumulation of fluorescent substrates, and by fluorescent substrate-antibiotic competition assay experiments. The purified protein exhibited host-specific in vitro hemolytic activity against various mammalian erythrocytes and apparent cytotoxicity in CHO-K1 cells. Recombinant Hly-OppA protein and an anti-Hly-OppA monoclonal antibody exhibited and neutralized the in vitro hemolytic activity, respectively, which further confirmed the hemolytic activity of the gene product. In addition, a V. furnissii hly-oppA knockout mutant caused less mortality than the wild-type strain when it was inoculated into BALB/c mice, indicating the virulence function of this protein. Finally, the in vitro hemolytic activity was also confirmed with homologous ATP-binding cassette-type transporter proteins from other Vibrio species.

* Corresponding author. Mailing address: Department of Biological Science and Technology, National Chiao Tung University, Hsin-Chu, Taiwan, 300, Republic of China. Phone: 886-3-5729287. Fax: 886-3-5725700. E-mail: tkwmll{at}mail.nctu.edu.tw

{triangledown} Published ahead of print on 14 September 2007.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, November 2007, p. 8215-8223, Vol. 189, No. 22
0021-9193/07/$08.00+0     doi:10.1128/JB.01039-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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